Alessandro Didonna University of California, San Francisco
48 protocols

Manjula Mummadisetti Rutgers university
11 protocols

Ralph Boettcher Max Planck Institute for Biochemistry
50 protocols

RAMESH KUDIRA RAMESH KUDIRA
10 protocols

Reviewer
Piyali Saha
  • Post-Doc, Stanford University
Research focus
  • Biochemistry
  • Cholesterol trafficking mechanism, Molecular Biology, Cell Biology, Biochemistry, Protein engineering, protein purification and characterization

Education

PhD, Indian Institute of Science, 2014

Lab information

Suzanne Pfeffer lab

Publications

1.Li X*, Saha P*, Li J*, Blobel G, Pfeffer SR. Clues to the mechanism of cholesterol transfer from the structure of NPC1 middle lumenal domain bound to NPC2. Proc Natl Acad Sci U S A. (2016) 113, 10079-84.
2. Li J*, Deffieu MS*, Lee PL*, Saha P, Pfeffer SR. Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells. Proc Natl Acad Sci U SA. (2015) 112, 14876-81.
3.Saha P*, Bhattacharyya S*, Kesavardhana S*, Miranda ER, Ali PS, Sharma D, Varadarajan R “Designed cyclic permutants of HIV-1 gp120: implications for envelope trimer structure and immunogen design”, Biochemistry (2012) 51, 1836-1847.
4. Saha P*, Barua B*, Bhattacharyya S, Balamurali MM, Schief WR, Baker D, Varadarajan R “Design and characterization of stabilized derivatives of human CD4D12 and CD4D1”, Biochemistry (2011) 50, 7891-7900.
5. Hu X, Saha P, Chen X, Kim D, Devarasetty M, Varadarajan R, Jin MM. Cell surface assembly of HIV gp41 six-helix bundles for facile, quantitative measurements of hetero-oligomeric interactions. J Am Chem Soc. (2012) 134, 14642-14645.
6. Rathore U, Saha P, Kesavardhana S, Kumar AA, Datta R, Devanarayanan S, Das R, Mascola JR, Varadarajan R. Glycosylation of the core of the HIV-1 envelope subunit protein gp120 is not required for native trimer formation or viral infectivity. J Biol Chem. (2017), 292, 10197-10219.
*Equal contribution
4 Protocols reviewed
Preparation of HeLa Total Membranes and Assay of Lipid-inhibition of Serine Palmitoyltransferase Activity
Serine palmitoyltranferase (SPT) is a pyridoxal 5′ phosphate (PLP)-dependent enzyme that catalyzes the first and rate-limiting step of de novo synthesis of sphingolipids. SPT activity is homeostatically regulated in response to increased ...
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Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells
Authors:  Irena Misiewicz-Krzeminska, Isabel Isidro and Norma C. Gutiérrez, date: 06/20/2019, view: 4001, Q&A: 0
Protein analysis in bone marrow samples from patients with multiple myeloma (MM) has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make ...
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