Ralph Boettcher Max Planck Institute for Biochemistry
35 protocols

1 protocol

Piyali Saha
  • Post-Doc, Stanford University
Research focus
  • Biochemistry
  • Cholesterol trafficking mechanism


PhD, Indian Institute of Science, 2014

Lab information

Suzanne Pfeffer lab


1.Li X*, Saha P*, Li J*, Blobel G, Pfeffer SR. Clues to the mechanism of cholesterol transfer from the structure of NPC1 middle lumenal domain bound to NPC2. Proc Natl Acad Sci U S A. (2016) 113, 10079-84.
2. Li J*, Deffieu MS*, Lee PL*, Saha P, Pfeffer SR. Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells. Proc Natl Acad Sci U SA. (2015) 112, 14876-81.
3.Saha P*, Bhattacharyya S*, Kesavardhana S*, Miranda ER, Ali PS, Sharma D, Varadarajan R “Designed cyclic permutants of HIV-1 gp120: implications for envelope trimer structure and immunogen design”, Biochemistry (2012) 51, 1836-1847.
4. Saha P*, Barua B*, Bhattacharyya S, Balamurali MM, Schief WR, Baker D, Varadarajan R “Design and characterization of stabilized derivatives of human CD4D12 and CD4D1”, Biochemistry (2011) 50, 7891-7900.
5. Hu X, Saha P, Chen X, Kim D, Devarasetty M, Varadarajan R, Jin MM. Cell surface assembly of HIV gp41 six-helix bundles for facile, quantitative measurements of hetero-oligomeric interactions. J Am Chem Soc. (2012) 134, 14642-14645.
6. Rathore U, Saha P, Kesavardhana S, Kumar AA, Datta R, Devanarayanan S, Das R, Mascola JR, Varadarajan R. Glycosylation of the core of the HIV-1 envelope subunit protein gp120 is not required for native trimer formation or viral infectivity. J Biol Chem. (2017), 292, 10197-10219.
*Equal contribution
2 Protocols reviewed
Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
Authors:  Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson, date: 12/05/2018, view: 338, Q&A: 0
To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type ...
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Deoxycholate Fractionation of Fibronectin (FN) and Biotinylation Assay to Measure Recycled FN Fibrils in Epithelial Cells
Authors:  Archana Varadaraj, Carina Magdaleno and Karthikeyan Mythreye, date: 08/20/2018, view: 647, Q&A: 0
Fibronectin (FN) is an extracellular matrix protein that is secreted by many cell types and binds predominantly to the cell surface receptor Integrin α5β1. Integrin α5β1 binding initiates the step-wise assembly of FN into fibrils, a process called ...
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