Alessandro Didonna University of California, San Francisco
35 protocols

Manjula Mummadisetti Rutgers university
11 protocols

Ralph Boettcher Max Planck Institute for Biochemistry
47 protocols

RAMESH KUDIRA RAMESH KUDIRA
3 protocols

Reviewer
Piyali Saha
  • Post-Doc, Stanford University
Research focus
  • Biochemistry
  • Cholesterol trafficking mechanism, Molecular Biology, Cell Biology, Biochemistry, Protein engineering, protein purification and characterization

Education

PhD, Indian Institute of Science, 2014

Lab information

Suzanne Pfeffer lab

Publications

1.Li X*, Saha P*, Li J*, Blobel G, Pfeffer SR. Clues to the mechanism of cholesterol transfer from the structure of NPC1 middle lumenal domain bound to NPC2. Proc Natl Acad Sci U S A. (2016) 113, 10079-84.
2. Li J*, Deffieu MS*, Lee PL*, Saha P, Pfeffer SR. Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells. Proc Natl Acad Sci U SA. (2015) 112, 14876-81.
3.Saha P*, Bhattacharyya S*, Kesavardhana S*, Miranda ER, Ali PS, Sharma D, Varadarajan R “Designed cyclic permutants of HIV-1 gp120: implications for envelope trimer structure and immunogen design”, Biochemistry (2012) 51, 1836-1847.
4. Saha P*, Barua B*, Bhattacharyya S, Balamurali MM, Schief WR, Baker D, Varadarajan R “Design and characterization of stabilized derivatives of human CD4D12 and CD4D1”, Biochemistry (2011) 50, 7891-7900.
5. Hu X, Saha P, Chen X, Kim D, Devarasetty M, Varadarajan R, Jin MM. Cell surface assembly of HIV gp41 six-helix bundles for facile, quantitative measurements of hetero-oligomeric interactions. J Am Chem Soc. (2012) 134, 14642-14645.
6. Rathore U, Saha P, Kesavardhana S, Kumar AA, Datta R, Devanarayanan S, Das R, Mascola JR, Varadarajan R. Glycosylation of the core of the HIV-1 envelope subunit protein gp120 is not required for native trimer formation or viral infectivity. J Biol Chem. (2017), 292, 10197-10219.
*Equal contribution
4 Protocols reviewed
Preparation of HeLa Total Membranes and Assay of Lipid-inhibition of Serine Palmitoyltransferase Activity
Serine palmitoyltranferase (SPT) is a pyridoxal 5′ phosphate (PLP)-dependent enzyme that catalyzes the first and rate-limiting step of de novo synthesis of sphingolipids. SPT activity is homeostatically regulated in response to increased ...
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Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells
Authors:  Irena Misiewicz-Krzeminska, Isabel Isidro and Norma C. Gutiérrez, date: 06/20/2019, view: 2942, Q&A: 0
Protein analysis in bone marrow samples from patients with multiple myeloma (MM) has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make ...
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