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Yu Murakami
Kyoto university, Japan
2 protocols
Akari Yonemura
1 protocol
Satoshi Ansai
1 protocol
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Masato Kinoshita
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2
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2 Protocols published
Medaka-microinjection with an Upright Microscope
Authors:
Yu Murakami
and
Masato Kinoshita
,
date:
02/05/2018,
view:
2881,
Q&A:
0
We described a simple method for microinjecting DNA/RNA/Protein solutions into medaka eggs under an upright microscope. Medaka is an excellent vertebrate model for reverse genetics, because of its daily spawning, short generation time, and egg ...
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Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins
Authors:
Yu Murakami
,
Satoshi Ansai
,
Akari Yonemura
and
Masato Kinoshita
,
date:
12/20/2017,
view:
3141,
Q&A:
0
This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus. This method makes it possible to determine the genotype of each individual simply by observing the fluorescence later ...
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