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Coauthors
Benedict Seddon Institute of Immunity and Transplantation, Univesirty College London, United Kingdom, United Kingdom,
2 protocols

Thea Hogan Institute of Immunity and Transplantation, Univesirty College London, United Kingdom, United Kingdom,
2 protocols

Andrew Yates
  • Institute of infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom, United Kingdom,
  • 2 Author merit
2 Protocols published
Generation of Busulfan Chimeric Mice for the Analysis of T Cell Population Dynamics
Authors:  Thea Hogan, Andrew Yates and Benedict Seddon, date: 12/20/2017, view: 980, Q&A: 0
This protocol was developed to generate chimeric mice in which T lymphocytes could be stratified by age on the basis of congenic marker expression. The conditioning drug busulfan is used to ablate host haematopoietic stem cells while leaving the ...
Analysing Temporal Dynamics of T Cell Division in vivo Using Ki67 and BrdU Co-labelling by Flow Cytometry
Authors:  Thea Hogan, Andrew Yates and Benedict Seddon, date: 12/20/2017, view: 1474, Q&A: 0
This protocol was developed to increase the richness of information available from in vivo T cell proliferation studies. DNA labelling techniques such as BrdU incorporation allow precise control of label administration and withdrawal, so ...