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Coauthors
Benedict Seddon
Institute of Immunity and Transplantation, Univesirty College London, United Kingdom, United Kingdom,
2 protocols
Thea Hogan
Institute of Immunity and Transplantation, Univesirty College London, United Kingdom, United Kingdom,
2 protocols
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Andrew Yates
Institute of infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom, United Kingdom,
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2 Protocols published
Generation of Busulfan Chimeric Mice for the Analysis of T Cell Population Dynamics
Authors:
Thea Hogan
,
Andrew Yates
and
Benedict Seddon
,
date:
12/20/2017,
view:
1410,
Q&A:
0
This protocol was developed to generate chimeric mice in which T lymphocytes could be stratified by age on the basis of congenic marker expression. The conditioning drug busulfan is used to ablate host haematopoietic stem cells while leaving the ...
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Analysing Temporal Dynamics of T Cell Division
in vivo
Using Ki67 and BrdU Co-labelling by Flow Cytometry
Authors:
Thea Hogan
,
Andrew Yates
and
Benedict Seddon
,
date:
12/20/2017,
view:
1996,
Q&A:
0
This protocol was developed to increase the richness of information available from
in vivo
T cell proliferation studies. DNA labelling techniques such as BrdU incorporation allow precise control of label administration and withdrawal, so ...
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