Dear Kate,

You can just order the sequences as reported by the Capsequm software.

An example:

For the Hba-1 promoter (DpnII fragment coordinates (mm9):chr11:32182969-32183821), we use the following oligos:

GATCCATGGTAGCACAGGGCAGCTAAGATGCAAGTCTGAAGGAGGAGTCTGGCGAGCTGCTCCTGCAGTTCCCTGGACCCAGAAGGATGAGCTAGCAGATTCACTTGAGCCAAAGGATTC

AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGTGAGAACAGGACCTTGATC

I hope that makes sense, please let me know if there’s anything else I can do to help.

Best wishes,
Marieke 7/27/2020 11:05:53 PM Reply