Andrea Puhar The Laboratory For Molecular Infection Medicine Sweden (MIMS) and Department of Molecular Biology, Umeå University, Sweden
5 protocols
David Cisneros Umeå University
31 protocols

Kabin Xie Huazhong Agricultural University
7 protocols

Ramón Cervantes-Rivera
  • Post-Doc, The Laboratory For Molecular Infection Medicine Sweden (MIMS) and Department of Molecular Biology, Umeå University, Umeå
  • 1 Author merit
Lab Protocol Hub
1 Protocol published
Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
Authors:  Ramón Cervantes-Rivera and Andrea Puhar, date: 09/20/2020, view: 369, Q&A: 0
Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are ...
1 Protocol reviewed
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria
Next generation high-throughput sequencing has enabled sensitive and unambiguous analysis of RNA populations in cells. Here, we describe a method for isolation and strand-specific sequencing of small RNA pools from bacteria that can be multiplexed ...
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