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A peer-reviewed protocol journal. No publication fee; no access fee.
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Coreviewers
David Cisneros
Umeå University
15 protocols
Kabin Xie
Huazhong Agricultural University
7 protocols
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Ramon Cervantes-Rivera
Post-Doc, Umeå University
Research focus
Molecular biology
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Lab information
Andrea Puhar Lab.
http://www.mims.umu.se/groups/andrea-puhar/puhar-group.html
1 Protocol reviewed
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria
Authors:
Sukrit Silas
,
Nimit Jain
,
Michael Stadler
,
Becky Xu Hua Fu
,
Antonio Sánchez-Amat
,
Andrew Z. Fire
and
Joshua Arribere
,
date:
02/20/2018,
view:
955,
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0
Next generation high-throughput sequencing has enabled sensitive and unambiguous analysis of RNA populations in cells. Here, we describe a method for isolation and strand-specific sequencing of small RNA pools from bacteria that can be multiplexed ...
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