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Andrea Puhar
MIMS and Department of Molecular Biology, Umeå University
5 protocols
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David Cisneros
Umeå University
33 protocols
Kabin Xie
Huazhong Agricultural University
7 protocols
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Ramón Cervantes-Rivera
Post-Doc, The Laboratory For Molecular Infection Medicine Sweden (MIMS) and Department of Molecular Biology, Umeå University, Umeå
Research focus
Biochemistry
Contributions
1
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Lab Protocol Hub
Puhar Lab
Lab information
Andrea Puhar Lab.
http://www.mims.umu.se/groups/andrea-puhar/puhar-group.html
1 Protocol published
Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria
Authors:
Ramón Cervantes-Rivera
and
Andrea Puhar
,
date:
09/20/2020,
view:
814,
Q&A:
0
Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are ...
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1 Protocol reviewed
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria
Authors:
Sukrit Silas
,
Nimit Jain
,
Michael Stadler
,
Becky Xu Hua Fu
,
Antonio Sánchez-Amat
,
Andrew Z. Fire
and
Joshua Arribere
,
date:
02/20/2018,
view:
6103,
Q&A:
0
Next generation high-throughput sequencing has enabled sensitive and unambiguous analysis of RNA populations in cells. Here, we describe a method for isolation and strand-specific sequencing of small RNA pools from bacteria that can be multiplexed ...
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