Francesca Cantini 1 protocol

Alexandre Bonvin Computational Structural Biology group, Bijvoet Center for Biomolecular Research, Faculty of Science Chemistry, Utrecht University, the Netherlands
2 protocols

Vincenzo Maione 1 protocol

Andrea Saponaro
  • Post-Doc, University of Milan
Research focus
  • Biophysics
  • 2 Author merit


University of Milan


• Santoro B., Hu L., Liu H., Saponaro A., Pian P., Piskorowski R. A., Moroni A., Siegelbaum S. A., “Tetratricopeptide Repeat-Containing Rab8b Interacting Protein (TRIP8b) regulates hyperpolarization-activated cyclic nucleotide-regulated 1 (HCN1) channel trafficking and gating through two distinct C-terminal interaction sites”, The Journal of Neuroscience, 2011, 31(11): 4074-4086 [doi:10.1523/JNEUROSCI.5707-10.2011].
• Smeazzetto S., Saponaro A., Young H.S., Moncelli M.R., Thiel G., “Structure-Function Relation of Phospholamban: Modulation of Channel Activity as a Potential Regulator of SERCA Activity”, PLoS One., 2013, 8(1):e52744 [doi: 10.1371/journal.pone.0052744].
• Hu L., Santoro B., Saponaro A., Liu H., Moroni A., Siegelbaum S. A., “Binding of the auxiliary subunit TRIP8b to HCN channels shift the mode of action of cAMP”, The Journal of General Physiology, 2013, 142(6): 599-612 [doi: 10.1085/jgp.201311013].
• Saponaro A., Pauleta S.R., Cantini F., Hu L., Matzapetakis M., Hammann C., Donadoni C., Thiel G., Banci L., Santoro B., Moroni A. “Structural basis for the mutual antagonism of cAMP and TRIP8b in regulating HCN channel function”, Proc Natl Acad Sci U S A., 2014, 111(40) :14577-82 [doi: 10.1073/pnas.1410389111].
• Weißgraeber S, Saponaro A., Thiel G., Hamacher K. “A reduced mechanical model for cAMP-modulated gating in HCN channels”. Sci Rep., 2017, 7:40168 [doi: 10.1038/srep40168].
• Saponaro A., Porro A., Chaves-Sanjuan A., Nardini M., Rauh O., Thiel G., Moroni A. “Fusicoccin Activates KAT1 Channels by Stabilizing Their Interaction with 14-3-3 Proteins”. Plant Cell. 2017, 29(10):2570-2580. [doi: 10.1105/tpc.17.00375].
• Saponaro A., Cantini F., Porro A., Bucchi A., DiFrancesco D., Maione V., Donadoni C., Introini B., Mesirca P., Mangoni M., Thiel G., Banci L., Santoro B., Moroni A. “A synthetic peptide that prevents cAMP regulation in mammalian Hyperpolarization-activated Cyclic Nucleotide-regulated (HCN) channels”. Elife. 2018, pii: e35753. doi: 10.7554/eLife.35753.
• Groß C., Saponaro A., Santoro B., Moroni A., Thiel G., Hamacher K. "Mechanical transduction of cytoplasmic-to-transmembrane-domain movements in a hyperpolarization-activated cyclic nucleotide–gated cation channel" J Biol Chem. 2018, pii: jbc.RA118.002139. doi: 10.1074/jbc.RA118.002139.
• Saponaro A. "Isothermal Titration Calorimetry: a Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution". 2018, Bio-protocol, Vol 8, Iss 15. doi: 10.21769/BioProtoc.2957.
• Alberio L., Locarno A., Saponaro A., Romano E., Bercier V., Albadri S., Del Bene F., Simeaoni F., Moleri S., Beltrame M., Pelucchi S., MarcellO E., Di Luca M., Kukovetz K., Boender AJ.,Luo S., Moutal A., Ji Y., Khanna R., Colecraft H.M., Thiel G., Tonini R., Moroni A. "A light-gated K+ channel for sustained neuronal inhibition in freely moving animals". Nat Methods. 2018 Nov;15(11):969-976. doi: 10.1038/s41592-018-0186-9.
• Porro A, Saponaro A, Gasparri F, Bauer D, Gross C, Pisoni M, Abbandonato G, Hamacher K, Santoro B, Thiel G, Moroni A. “The HCN domain couples voltage gating and cAMP response in hyperpolarization-activated cyclic nucleotide-gated channels”. Elife. 2019 Nov 26;8. pii: e49672. doi: 10.7554/eLife.49672.
• Brocca P, Saponaro A, Introini B, Rondelli V, Pannuzzo M, Raciti D, Corti M, Raudino A. “Protein Adsorption at the Air-Water Interface by a Charge Sensing Interferometric Technique”. Langmuir. 2019 Dec 10;35(49):16087-16100. doi: 10.1021/acs.langmuir.9b02201. Epub 2019 Nov 19.
• Kukovetz K, Hertel B, Schvarcz CR, Saponaro A, Manthey M, Burk U, Greiner T, Steward GF, Van Etten JL,Moroni A, Thiel G, Rauh O. “A Functional K + Channel from Tetraselmis Virus 1, a Member of the Mimiviridae”. Viruses, 2020 12(10):1107. doi: 10.3390/v12101107.
• Nardone V, Chaves-Sanjuan A, Lapi M, Airoldi C, Saponaro A, Pasqualato S, Dolfini D, Camilloni C, Bernardini A, Gnesutta N, Mantovani R, Nardini M. “Structural Basis of Inhibition of the Pioneer Transcription Factor NF-Y by Suramin”. Cells. 2020 9(11):2370. doi: 10.3390/cells9112370.
• Porro A, Binda A, Pisoni M, Donadoni C, Rivolta I, Saponaro A. “Rational design of a mutation to investigate the role of the brain protein TRIP8b in limiting the cAMP response of HCN channels in neurons”. J General Physiol. 2020, doi: 10.1085/jgp.202012596.
• Saponaro A, Maione V, Bonvin A. M. J. J., Cantini F. “Understanding Docking Complexes of Macromolecules Using HADDOCK: The Synergy between Experimental Data and Computations”. Bio-protocol, 2020, Vol 10, Iss 20. doi: 10.21769/BioProtoc.3793.
• Porro A, Thiel G, Moroni A, Saponaro A. “cyclic AMP Regulation and Its Command in the Pacemaker Channel HCN4”. Front. Physiol. 2020, doi: 10.3389/fphys.2020.00771.
2 Protocols published
Understanding Docking Complexes of Macromolecules Using HADDOCK: The Synergy between Experimental Data and Computations
Authors:  Andrea Saponaro, Vincenzo Maione, Alexandre M. J. J. Bonvin and Francesca Cantini, date: 10/20/2020, view: 982, Q&A: 0
This protocol illustrates the modelling of a protein-peptide complex using the synergic combination of in silico analysis and experimental results. To this end, we use the integrative modelling software HADDOCK, which possesses the powerful ...
Isothermal Titration Calorimetry: A Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution
Author:  Andrea Saponaro, date: 08/05/2018, view: 17061, Q&A: 0
This protocol can be applied to analyze the direct interaction between a soluble protein and a target ligand molecule using Isothermal Titration Calorimetry (ITC, Malvern). ITC allows the biophysical characterization of binding between label-free, ...
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