Francesca Cantini 1 protocol

Alexandre Bonvin Computational Structural Biology group, Bijvoet Center for Biomolecular Research, Faculty of Science Chemistry, Utrecht University, the Netherlands
2 protocols

Vincenzo Maione 1 protocol

Andrea Saponaro
  • Post-Doc, Milan Milan
Research focus
  • Biochemistry
  • 2 Author merit


PhD Degree in Biomolecular sciences , University of Milan, Italy, 2014

Current position

Researcher, Biosciences, University of Milan, Italy


  1. Saponaro, A., Cantini, F., Porro, A., Bucchi, A., DiFrancesco, D., Maione, V., Donadoni, C., Introini, B., Mesirca, P., Mangoni, M. E., Thiel, G., Banci, L., Santoro, B. and Moroni, A. (2018). A synthetic peptide that prevents cAMP regulation in mammalian hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. Elife 7.
  2. Gross, C., Saponaro, A., Santoro, B., Moroni, A., Thiel, G. and Hamacher, K. (2018). Mechanical transduction of cytoplasmic-to-transmembrane-domain movements in a hyperpolarization-activated cyclic nucleotide-gated cation channel. J Biol Chem.
  3. Weissgraeber, S., Saponaro, A., Thiel, G. and Hamacher, K. (2017). A reduced mechanical model for cAMP-modulated gating in HCN channels. Sci Rep 7: 40168.
  4. Saponaro, A., Porro, A., Chaves-Sanjuan, A., Nardini, M., Rauh, O., Thiel, G. and Moroni, A. (2017). Fusicoccin activates KAT1 channels by stabilizing their interaction with 14-3-3 proteins. Plant Cell 29(10): 2570-2580.
  5. Saponaro, A., Pauleta, S. R., Cantini, F., Matzapetakis, M., Hammann, C., Donadoni, C., Hu, L., Thiel, G., Banci, L., Santoro, B. and Moroni, A. (2014). Structural basis for the mutual antagonism of cAMP and TRIP8b in regulating HCN channel function. Proc Natl Acad Sci U S A 111(40): 14577-14582.
  6. Smeazzetto, S., Saponaro, A., Young, H. S., Moncelli, M. R. and Thiel, G. (2013). Structure-function relation of phospholamban: modulation of channel activity as a potential regulator of SERCA activity. PLoS One 8(1): e52744.
  7. Hu, L., Santoro, B., Saponaro, A., Liu, H., Moroni, A. and Siegelbaum, S. (2013). Binding of the auxiliary subunit TRIP8b to HCN channels shifts the mode of action of cAMP. J Gen Physiol 142(6): 599-612.
  8. Santoro, B., Hu, L., Liu, H., Saponaro, A., Pian, P., Piskorowski, R. A., Moroni, A. and Siegelbaum, S. A. (2011). TRIP8b regulates HCN1 channel trafficking and gating through two distinct C-terminal interaction sites. J Neurosci 31(11): 4074-4086.
2 Protocols published
Understanding Docking Complexes of Macromolecules Using HADDOCK: The Synergy between Experimental Data and Computations
Authors:  Andrea Saponaro, Vincenzo Maione, Alexandre M. J. J. Bonvin and Francesca Cantini, date: 10/20/2020, view: 701, Q&A: 0
This protocol illustrates the modelling of a protein-peptide complex using the synergic combination of in silico analysis and experimental results. To this end, we use the integrative modelling software HADDOCK, which possesses the powerful ...
Isothermal Titration Calorimetry: A Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution
Author:  Andrea Saponaro, date: 08/05/2018, view: 16312, Q&A: 0
This protocol can be applied to analyze the direct interaction between a soluble protein and a target ligand molecule using Isothermal Titration Calorimetry (ITC, Malvern). ITC allows the biophysical characterization of binding between label-free, ...
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