Ortrun Scheid Gregor Mendel Institute of Molecular Plant Biology, Austria
2 protocols
Agnieszka Zienkiewicz Georg August University
14 protocols

Aleksandr Gavrin Sainsbury Laboratory University of Cambridge
3 protocols

Amey Redkar Department of Genetics, University of Cordoba
16 protocols

Ana Espinosa-Ruiz Instituto de Biología Molecular y Celular de Plantas
1 protocol

Editor
Marisa Rosa
  • Editor, Bio-protocol.org
Research focus
  • Plant science
  • 2 Author merit

Education

PhD, University of Vienna, 2012

Publications

https://scholar.google.com/citations?user=tRx8IQ0AAAAJ&hl=en
•Rosa, M., et al. (2017) The Maize MID-COMPLEMENTING ACTIVITY Homolog CELL NUMBER REGULATOR13/NARROW ODD DWARF Coordinates Organ Growth and Tissue Patterning. The Plant Cell 29: 1-17

•Rosa, M., et al. (2013) The Arabidopsis SWR1 chromatin-remodeling complex is important for DNA repair, somatic recombination, and meiosis. The Plant Cell 5: 1990-2000

•Pecinka, A., Rosa, M., et al. (2009) Transgenerational stress memory is not a general response in Arabidopsis. PloS one 4: e5202

•Pecinka, A., Dinh, H.Q., Baubec, T., Rosa, M., et al. (2010) Epigenetic regulation of repetitive elements is attenuated by prolonged heat stress in Arabidopsis. The Plant Cell 22: 3118–3129

•Erilova, A., Brownfield, L., Exner, V., Rosa, M., et al. (2009) Imprinting of the Polycomb group gene MEDEA serves as a ploidy sensor in Arabidopsis. PLoS Genet. 25: e1000663

•Caperta, A.D., Rosa, M., et al. (2008) Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant mitosis and meiosis. Cytogenet Genome Res. (2008) 122: 73–79
2 Protocols published
Measuring Homologous Recombination Frequency in Arabidopsis Seedlings
Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 8377, Q&A: 0
Somatic homologous recombination (SHR) is a major pathway of DNA double-strand break (DSB) repair, in which intact homologous regions are used as a template for the removal of lesions. Its frequency in plants is generally low, as most DSB are ...
DNA Damage Sensitivity Assays with Arabidopsis Seedlings
Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 7402, Q&A: 0
We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a ...
7 Protocols reviewed
RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis
Authors:  Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 2966, Q&A: 0
RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
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53 Protocols edited
Transient Transformation of Maize BMS Suspension Cells via Particle Bombardment
Authors:  Liang-Zi Zhou and Thomas Dresselhaus, date: 12/05/2019, view: 59, Q&A: 0
Maize is one of the most important crop species and serves also as a model plant for grass research. A major bottleneck in maize research is stable transformation, which is both time and cost consuming, but also a technical challenge for most labs ...
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A Simple Protocol for Informative Visualization of Enriched Gene Ontology Terms
Authors:  Titouan Bonnot, Morgane B. Gillard and Dawn H. Nagel, date: 11/20/2019, view: 257, Q&A: 0
In genome-scale datasets, Gene Ontology (GO) enrichment is a common analysis to highlight functions over-represented or under-represented in a subset of differentially expressed genes to elucidate the biological significance of the results. However, ...
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