Ortrun Scheid Gregor Mendel Institute of Molecular Plant Biology, Austria
2 protocols
Agnieszka Zienkiewicz Georg August University
14 protocols

Aleksandr Gavrin Sainsbury Laboratory University of Cambridge
3 protocols

Amey Redkar Department of Genetics, University of Cordoba
15 protocols

Ana Espinosa-Ruiz Instituto de Biología Molecular y Celular de Plantas
1 protocol

Editor
Marisa Rosa
  • Editor, Bio-protocol.org
Research focus
  • Plant science
  • 2 Author merit

Education

PhD, University of Vienna, 2012

Publications

https://scholar.google.com/citations?user=tRx8IQ0AAAAJ&hl=en
•Rosa, M., et al. (2017) The Maize MID-COMPLEMENTING ACTIVITY Homolog CELL NUMBER REGULATOR13/NARROW ODD DWARF Coordinates Organ Growth and Tissue Patterning. The Plant Cell 29: 1-17

•Rosa, M., et al. (2013) The Arabidopsis SWR1 chromatin-remodeling complex is important for DNA repair, somatic recombination, and meiosis. The Plant Cell 5: 1990-2000

•Pecinka, A., Rosa, M., et al. (2009) Transgenerational stress memory is not a general response in Arabidopsis. PloS one 4: e5202

•Pecinka, A., Dinh, H.Q., Baubec, T., Rosa, M., et al. (2010) Epigenetic regulation of repetitive elements is attenuated by prolonged heat stress in Arabidopsis. The Plant Cell 22: 3118–3129

•Erilova, A., Brownfield, L., Exner, V., Rosa, M., et al. (2009) Imprinting of the Polycomb group gene MEDEA serves as a ploidy sensor in Arabidopsis. PLoS Genet. 25: e1000663

•Caperta, A.D., Rosa, M., et al. (2008) Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant mitosis and meiosis. Cytogenet Genome Res. (2008) 122: 73–79
2 Protocols published
Measuring Homologous Recombination Frequency in Arabidopsis Seedlings
Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 8019, Q&A: 0
Somatic homologous recombination (SHR) is a major pathway of DNA double-strand break (DSB) repair, in which intact homologous regions are used as a template for the removal of lesions. Its frequency in plants is generally low, as most DSB are ...
DNA Damage Sensitivity Assays with Arabidopsis Seedlings
Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 7094, Q&A: 0
We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a ...
7 Protocols reviewed
RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis
Authors:  Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 2556, Q&A: 0
RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
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51 Protocols edited
Isolation and Efficient Maize Protoplast Transformation
Authors:  Lina Gomez-Cano, Fan Yang and Erich Grotewold, date: 08/20/2019, view: 64, Q&A: 0
Establishing plant gene function and the study of gene expression regulation often require that genes (or gene variants) are introduced into plants and their activity somehow assayed. Some plants (e.g., Arabidopsis) are easily ...
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Quantifying MAMP-induced Production of Reactive Oxygen Species in Sorghum and Maize
Plants have the ability to recognize microbe-associated molecular patterns (MAMPs) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, a ...
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