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Coauthors
Ortrun Scheid Gregor Mendel Institute of Molecular Plant Biology, Austria
2 protocols
Coreviewers
Amey Redkar Department of Genetics, University of Cordoba
9 protocols

Ana Espinosa-Ruiz Instituto de Biología Molecular y Celular de Plantas
1 protocol

Rekha Warrier Scientist
1 protocol

Renate Weizbauer Carnegie Institution for Science
39 protocols

Editor
Marisa Rosa
  • University of California, Berkeley
Research focus
  • Plant science
  • 2 Author merit

Education

Ph.D. in Molecular Biology, Gregor Mendel Institute, University of Vienna, Austria, 2012

Current position

Postdoctoral scholar in Developmental Plant Biology, University of California Berkeley, Berkeley, USA (04/2013-present)

Publications

  1. Rosa, M., Von Harder, M., Cigliano, R. A., Schlogelhofer, P. and Mittelsten Scheid, O. (2013). The Arabidopsis SWR1 chromatin-remodeling complex is important for DNA repair, somatic recombination, and meiosis. Plant Cell 25(6): 1990-2001.
  2. Pecinka, A., Rosa, M., Schikora, A., Berlinger, M., Hirt, H., Luschnig, C. and Mittelsten Scheid, O. (2009). Transgenerational stress memory is not a general response in Arabidopsis. PLoS One 4(4): e5202.
  3. Pecinka, A., Dinh, H. Q., Baubec, T., Rosa, M., Lettner, N. and Mittelsten Scheid, O. (2010). Epigenetic regulation of repetitive elements is attenuated by prolonged heat stress in Arabidopsis. Plant Cell 22(9): 3118-3129.
  4. Erilova, A., Brownfield, L., Exner, V., Rosa, M., Twell, D., Mittelsten Scheid, O., Hennig, L. and Kohler, C. (2009). Imprinting of the polycomb group gene MEDEA serves as a ploidy sensor in Arabidopsis. PLoS Genet 5(9): e1000663.
  5. Caperta, A. D., Rosa, M., Delgado, M., Karimi, R., Demidov, D., Viegas, W. and Houben, A. (2008). Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant mitosis and meiosis. Cytogenet Genome Res 122(1): 73-79.
2 Protocols published
Measuring Homologous Recombination Frequency in Arabidopsis Seedlings
Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 6294, Q&A: 0
Somatic homologous recombination (SHR) is a major pathway of DNA double-strand break (DSB) repair, in which intact homologous regions are used as a template for the removal of lesions. Its frequency in plants is generally low, as most DSB are ...
DNA Damage Sensitivity Assays with Arabidopsis Seedlings
Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 5508, Q&A: 0
We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a ...
2 Protocols reviewed
RNA Immunoprecipitation (RIP) Sequencing of Pri-miRNAs Associated with the Dicing Complex in Arabidopsis
Authors:  Ziwei Li, Songxiao Zhong, Wenye Wu and Binglian Zheng, date: 07/05/2018, view: 338, Q&A: 0
RNA immunoprecipitation (RIP) is an antibody-based technique used to map in vivo RNA-protein interactions. DBR1, an RNA debranching enzyme, is responsible for the debranching of lariat RNA, for the degradation and turnover of lariat RNAs. ...
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Determination of H+-ATPase Activity in Arabidopsis Guard Cell Protoplasts through H+-pumping Measurement and H+-ATPase Quantification
Authors:  Shota Yamauchi and Ken-ichiro Shimazaki, date: 12/20/2017, view: 1590, Q&A: 0
The opening of stomata in plants in response to blue light is driven by the plasma membrane H+-ATPase in guard cells. To evaluate the activation of the H+-ATPase in vivo, we can use H+-pumping by guard cells ...
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1 Protocol edited
Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana
Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its’ inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic ...
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