You should have a filter set to collect the emission of luciferase at luciferase emission wavelength (465 bandwidth 25) and the emission of YFP at the emission of YFP wavelength ( 535, bandwidth 25). So when you add coeleterazine h to excite the luciferase and the collect the emission of YFP.
Basically emission measured in luminescence model
2020-08-10 08:25:07