Matthew Shurtleff 1 protocol

Kanika Bajaj Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
1 protocol

Satoshi Baba Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
1 protocol

Randy Schekman
  • Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
Research focus
  • Biochemistry
  • 2 Author merit
2 Protocols published
Buoyant Density Fractionation of Small Extracellular Vesicle Sub-populations Derived from Mammalian Cells
Authors:  Morayma M. Temoche-Diaz, Matthew J. Shurtleff and Randy Schekman, date: 08/05/2020, view: 876, Q&A: 0
Small extracellular vesicles (sEVs) encompass a variety of distinct vesicles that are secreted to the extracellular space. Many methodologies currently used for EV isolation (e.g., differential ultracentrifugation concluding in a high-speed ...
Cell-free Generation of COPII-coated Procollagen I Carriers
Authors:  Lin Yuan, Satoshi Baba, Kanika Bajaj and Randy Schekman, date: 11/20/2017, view: 4981, Q&A: 0
The aim of this protocol is to generate COPII-coated procollagen I (PC1) carriers in a cell-free reaction. The COPII-coated PC1 carriers were reconstituted from donor membrane, cytosol, purified recombinant COPII proteins, and nucleotides. This ...
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