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Randy Schekman
Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
1 protocol
Satoshi Baba
Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
1 protocol
Lin Yuan
Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
1 protocol
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Kanika Bajaj
Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, USA, USA,
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1 Protocol published
Cell-free Generation of COPII-coated Procollagen I Carriers
Authors:
Lin Yuan
,
Satoshi Baba
,
Kanika Bajaj
and
Randy Schekman
,
date:
11/20/2017,
view:
2364,
Q&A:
0
The aim of this protocol is to generate COPII-coated procollagen I (PC1) carriers in a cell-free reaction. The COPII-coated PC1 carriers were reconstituted from donor membrane, cytosol, purified recombinant COPII proteins, and nucleotides. This ...
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