Jitendra Thakur Plant Mediator Lab, National Institute of Plant Genome Research , INDIA, INDIA,
1 protocol

Shubhashis Das Plant Mediator Lab, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, India, India,
1 protocol
David Cisneros Umeå University
23 protocols

Dušan Veličković Pacific Northwest National Laboratory
3 protocols

Renate Weizbauer Carnegie Institution for Science
41 protocols

Sesha Lakshmi Paluri Yale University
3 protocols

Manish Kumar Patel
  • Post-Doc, Plant Mediator Lab, National Institute of Plant Genome Research , INDIA, INDIA,
Research focus
  • Biochemistry
  • Transcriptomics and Metabolomics, Molecular Biology, Abiotic Stress, Plant Tissue Culture
  • 1 Author merit


P. hd, CSIR-CSMCRI, 2016

Lab information

Jitendra Kumar Thakur,s Lab


1. Tiwari V, Patel M K, Chaturvedi A K, Mishra A, Jha B (2019) Cloning and functional characterization of the Na+/H+ antiporter (NHX1) gene promoter from an extreme halophyte Salicornia brachiata. Gene 683, 233-242.

2. Patel M K, Das S, Thakur J K (2018) GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues. Bio-protocol 8, 1-9.

3. Patel M K, Tanna B, Mishra A, Jha B (2018) Physicochemical characterization, antioxidant and anti-proliferative activities of a polysaccharide extracted from psyllium (P. ovata) leaves. International Journal of Biological Macromolecules. 118, 976-987

4. Patel M K, Mishra A, Jha B (2016) Non-targeted metabolite profiling and scavenging activity unveil the nutraceutical potential of psyllium (Plantago ovata Forsk). Frontiers in Plant Science 7: 431

5. Patel M K, Joshi M, Mishra A, Jha B (2015) Ectopic expression of SbNHX1 gene in transgenic castor (Ricinus communis L.) enhances salt stress by modulating physiological process. Plant Cell Tissue Organ Culture (PCTOC) 122 (2): 477-490. (4/1 – Q1).

6. Tiwari V, Patel M K, Chaturvedi AK, Mishra A, Jha B (2016) Functional characterization of the Tau Class Glutathione-S-Transferases gene (SbGSTU) promoter of Salicornia brachiata under salinity and osmotic stress. PLoS ONE 11(2): e0148494 (5/2 – Q1).

7. Pandey S, Patel M K, Mishra A, Jha B (2016) An in planta transformed cumin (Cuminum cyminum L.) plants, overexpressing the SbNHX1 gene showed enhanced salt endurance. PLoS ONE 11(7): e0159349 (4/2 – Q1).

8. Jaiswar S, Balar N, Kumar R, Patel M K, Chauhana P S (2016) Morphological and molecular characterization of newly isolated microalgal strain Neochloris aquatica and its lipid productivity. Biocatalysis and Agricultural Biotechnology 9: 108–112 (5/4 – Q1).

9. Mishra A, Patel M K, Jha B (2015) Non-targated metabolomics and scavenging activity of reactive oxygen species reveal the potential of Salicornia brachiata as a functional food. Journal of Functional Foods 13: 21-3 (3/2 – Q1).

10. Pandey S, Patel M K, Mishra A, Jha B (2015) Physio-biochemical composition and untargeted metabolomics of cumin (Cuminum cyminum L.) make it promising functional food and help in mitigating salinity stress. PLoS ONE 10(12): e0144469 (4/2 – Q1).

11. Chaturvedi A K, Patel M K, Mishra A, Tiwari V, Jha B (2014) The SbMT-2 gene from a halophyte confers abiotic stress tolerance and modulates ROS scavenging in transgenic tobacco. PLoS ONE 9 (10): e111379 (5/2 – Q1).

12. Pandey S, Mishra A, Patel M K, Jha B (2013) An efficient method for Agrobacterium-mediated genetic transformation and plant regeneration in cumin (Cuminum cyminum L.). Applied Biochemistry and Biotechnology 171 (1): 1-9 (4/3 – Q2).

13. Patel M K, Mishra A, Jha B (2016) Untargeted Metabolomics of Halophytes. In: Kim S (Ed.) Marine Omics: Principles and Applications, CRC Press, Boca Raton, Florida, USA, 309–325 (ISBN 9781482258202).
1 Protocol published
GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues
Authors:  Manish Kumar Patel, Shubhashis Das and Jitendra Kumar Thakur, date: 09/20/2018, view: 855, Q&A: 0
Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and ...
2 Protocols reviewed
Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice
Short-Chain Fatty Acids (SCFAs) are a product of the fermentation of resistant starches and dietary fibers by the gut microbiota. The most important SCFA are acetate (C2), propionate (C3) and butyrate (C4). These metabolites are formed and absorbed ...
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Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method
Authors:  Tomáš Zavřel, Petra Očenášová, Maria A. Sinetova and Jan Červený, date: 08/05/2018, view: 998, Q&A: 0
This is a protocol for quantitative determination of storage and total carbohydrates in algae and cyanobacteria. The protocol is simple, fast and sensitive and it requires only few standard chemicals. Great advantage of this protocol is that both ...
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