Hi,
if the DNA is extracted properly, then the A260/280 value will be 1.8. If the absorbance value crosses 2, then there is protein contamination or RNA contamination. To reduce the absorbance value to 1.8 and decrease protein/RNA contamination, just add 5-10ul of MilliQ to the DNA. Or, repeat DNA extraction again from the scratch
6/25/2019 9:15:23 PM Reply