Cristina Risco Cell Structure Laboratory, National Center for Biotechnology , Spain, Spain,
1 protocol

Isabel Fernandez de Castro
  • Post-Doc, Cell Structure Laboratory, National Center for Biotechnology , Spain, Spain,
Research focus
  • Advanced light and electron microscopy methods to investigate the biogenesis and architecture of viral factories assembled by RNA viruses.
  • 1 Author merit


PhD, Complutense University of Madrid, 2014

Lab information

Cristina´s Risco Lab investigates the assembly and architecture of virus factories, replication complexes and morphogenetic pathways of several RNA viruses that are important human pathogens.


• Isabel Fernández de Castro & Cristina Risco. Metal-tagging Transmission Electron Microscopy for Localisation of Viral Replication Compartments. Bioprotocol. (2018) Accepted. In press.
• Raquel Tenorio, Isabel Fernández de Castro, Jonathan J. Knowlton, Paula F. Zamora, Bernardo A. Mainou, Terence S. Dermody, and Cristina Risco. Reovirus σNS and μNS proteins remodel the endoplasmic reticulum to build replication neo-organelles. PNAS (2018). Submitted.
• Jonathan Knowlton , Isabel Fernandez De Castro , Alison Ashbrook , Daniel Gestaut , Paula Zamora , Joshua Bauer , James Forrest , Judith Frydman , Cristina Risco. The TRiC Chaperonin Controls Reovirus Replication through Outer-Capsid Folding. Nature Microbiology (2018). Accepted. In press.
• Isabel Fernández de Castro Martin, Guillaume Fournier, Martin Sachse,
Javier Pizarro-Cerda, Cristina Risco, Nadia Naffakh. A new class of Rab11-
dependent vesicles transports influenza virus genome from a modified
endoplasmic reticulum to the plasma membrane. Nature Communications (2017). DOI: 10.1038/s41467-017-01557-6.
• Fernández de Castro, I., Fernández, J. J., Barajas, D., Nagy, P. D., Risco, C.
Three-dimensional imaging of the intracellular assembly of a functional viral RNA replicase complex. J. Cell Sci. (2017) doi:10.1242/jcs.181586.
Corresponding author.
• Fernández de Castro, I., Tenorio, R., Risco, C. Virus assembly factories in a lipid world. Curr Opin Virol. 18:20-26. (2016). Corresponding author.
• Nawaz-Ul-Rehman MS, Prasanth KR, Xu K, Sasvari Z, Kovalev N, de Castro Martín IF, Barajas D, Risco C, Nagy PD. Viral Replication Protein Inhibits Cellular Cofilin Actin Depolymerization Factor to Regulate the Actin Network and Promote Viral Replicase Assembly. PLoS Pathog. 12(2):e1005440. (2016).
• Prasanth KR, Kovalev N, de Castro Martín IF, Baker J, Nagy PD. Screening a yeast library of temperature-sensitive mutants reveals a role for actin in tombusvirus RNA recombination. Virology. 13;489:233-242. (2016).
• Kovalev N, Martín IF, Pogany J, Barajas D, Pathak K, Risco C, Nagy PD. Role of viral RNA and co-opted cellular ESCRT-I and ESCRT-III factors in formation of tombusvirus spherules harboring the tombusvirus replicase. J Virol. 20. pii: JVI.02775-15. (2016).
• Risco C., Fernández de Castro I., Sanz-Sánchez L., Narayan K., Grandinetti G., Subramaniam S. Three-Dimensional Imaging of Viral Infections. Annual Review of Virology. Vol. 1: 453-473. (2014).
• Fernández de Castro, I., Sanz-Sánchez, L., & Risco, C. Metallothioneins for Correlative Light and Electron Microscopy. In: Correlative Light and Electron Microscopy. Methods in Cell Biology, vol 124, Chapter 3, pp. 55-70. Elsevier,ISSN 0091-679X. (2014).
• Fernández de Castro I., Zamora Paula F., Ooms L., Fernández J.J., M.-H. La C., Mainou Bernardo A., Dermody Terence S., Risco C. Reovirus forms neoorganelles for progeny particle assembly within reorganized cell membranes. MBio. 5(1). pii: e00931-13. (2014).
• Barajas* D., Fernández de Castro* I., Pogany J., Risco C., Nagy P.D.
Noncanonical role for the host Vps4 AAA+ ATPase ESCRT protein in the
formation of Tomato bushy stunt virus replicase. PLoS Pathog. 10(4):
e1004087. (*) Equal contribution. (2014).
• Barajas D., Xu K., Fernández de Castro Martín I., Sasvari Z., Brandizzi F.,
Risco C., Nagy P.D. Co-opted Oxysterol-Binding ORP and VAP Proteins
Channel Sterols to RNA Virus Replication Sites via Membrane Contact Sites. PLoS Pathog. 10(10): e1004388. (2014).
• Fernández de Castro, I. & Risco, C. Imaging RNA virus replication
assemblies: bunyaviruses and reoviruses. Future Virol. 9(12), 1089–1104. (2014). Corresponding author.
• Li B., Wang Q., Pan X., Fernández de Castro I., Sun Y., Guo Y., Tao X.,
Risco C., Sui S.F., Lou Z. Bunyamwera virus possesses a distinct
nucleocapsid protein to facilitate genome encapsidation. Proc Natl Acad Sci US A. 110(22):9048-53. (2013).
• Fernández de Castro, I., Volontè, L., Risco, C. Virus factories: biogenesis andstructural design. Cell Microbiol. Special issue “Cell Biology of Microbes” 15(1):24-34. (2013).
• Risco, C., Fernández de Castro, I. Virus Morphogenesis in the Cell: Methods and Observations. M.G. Mateu (ed.). Chapter 14 in: “Structure and Physics of Viruses: An Integrated Textbook”, Subcellular Biochemistry 68, DOI 10.1007/978-94-007-6552-8_14, Springer SBM Dordrecht. (2013).
• Guzmán-Fulgencio, M., Berenguer, J., Fernández de Castro, I., Micheloud, D., López, J.C., Cosín, J., Miralles, P., Lorente, R., Aldamiz-Echevarría, T., Muñoz- Fernández, M.A., Resino, S. Sustained virological response to interferon-α plus ribavirin decreases inflammation and endothelial dysfunction markers in HIV/HCV co-infected patients. J Antimicrob Chemother. 66: 645–649. (2011).
• Guzmán-Fulgencio, M., Berenguer, J., García-Álvarez, M., Micheloud, D.,
López, J. C., Cosín, J., Fernández de Castro, I., Catalán, P., Miralles, P.,
Resino, S. Soluble Fas and Fas ligand in HIV/HCV coinfected patients and
impact of HCV therapy. Eur J Clin Microbiol Infect Dis. 30 (10):1231-1221.
• Fernández de Castro, I., Berenguer, J., Micheloud, D., Catalán, P., Miralles, P., Álvarez, E., López, J.C., Cosín, J., Resino, S. Serum levels of adipokines in HIV/HCV co-infected patients and their association with insulin resistance and liver disease severity. J Infect. 61(6):499-501. (2010).
• Fernández de Castro, I., Guzmán-Fulgencio, M., García-Álvarez, M., Resino, S. First evidence of a pro-inflamatory response to severe infection with influenza virus H1N1. Critical Care. 14: 115. (2010).
• Fernández de Castro, I., Micheloud, D., Berenguer, J., Guzmán-Fulgencio, M., Catalán, P., Miralles, P., Álvarez, E., López, J.C., Cosín, J., Lorente, R., Muñoz-Fernández M.A., Resino, S. Hepatitis C virus infection is associated with endothelial dysfunction in HIV/HCV coinfected patients. AIDS. 24: 2059-2067.

1 Protocol published
Metal-tagging Transmission Electron Microscopy for Localisation of Tombusvirus Replication Compartments in Yeast
Authors:  Isabel Fernández de Castro and Cristina Risco, date: 04/20/2018, view: 172, Q&A: 0
Positive-stranded (+) RNA viruses are intracellular pathogens in humans, animals and plants. To build viral replicase complexes (VRCs) viruses manipulate lipid flows and reorganize subcellular membranes. Redesigned membranes concentrate viral and ...