Home
About Us
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
Archive
My bio page
Edit user profile
Signature
Reset the password
Home
About Us
For Authors
Submission Procedure
Preparation Guidelines
Submit Manuscript
Editorial Process
Editorial Criteria
Publishing Ethics
Competing Interests
Copyright & Permissions
Archive
A peer-reviewed protocol journal. No publication fee; no access fee.
Search
Overview
Published
Edited
Reviewed
Following
Feedback
Q&A
Tetsuya Okajima
Nagoya University, Japan
4 protocols
Mitsutaka Ogawa
4 protocols
Entzu Wan
2 protocols
Ailing Zhang
1 protocol
View all coauthors >
Shogo Sawaguchi
Research focus
Developmental biology
Contributions
2
Author merit
Reviewer merit
Editor merit
2 Protocols published
Isolation of Murine Brain and Lung Microvascular Endothelial Cells
Authors:
Ailing Zhang
,
Shogo Sawaguchi
,
Entzu Wan
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
08/20/2018,
view:
3411,
Q&A:
0
This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak
et al.
, 2010; Ruck
et al.
, 2014). We have used the protocol to analyze ...
More >
Protocol for Notch-ligand Binding Assays Using Dynabeads
Authors:
Shogo Sawaguchi
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
10/20/2017,
view:
5876,
Q&A:
0
This protocol describes how to measure interaction between Notch receptors and their ligands by cell-based assay using Dynabeads. We have used the protocol to determine binding capacity between Notch1-transfected HEK293T cells and ligand-coated ...
More >
following
More protocols >
0 Protocol feedback
Find out more
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.