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Coauthors
Tetsuya Okajima Nagoya, Japan
2 protocols

Mitsutaka Ogawa 2 protocols

Entzu Wan 1 protocol

Ailing Zhang 1 protocol

Shogo Sawaguchi
  • 2 Author merit
2 Protocols published
Isolation of Murine Brain and Lung Microvascular Endothelial Cells
Authors:  Ailing Zhang, Shogo Sawaguchi, Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 08/20/2018, view: 112, Q&A: 0
This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak et al., 2010; Ruck et al., 2014). We have used the protocol to analyze ...
Protocol for Notch-ligand Binding Assays Using Dynabeads
Authors:  Shogo Sawaguchi, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2017, view: 2135, Q&A: 0
This protocol describes how to measure interaction between Notch receptors and their ligands by cell-based assay using Dynabeads. We have used the protocol to determine binding capacity between Notch1-transfected HEK293T cells and ligand-coated ...