Shogo Sawaguchi —BIO-PROTOCOL

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Tetsuya Okajima Nagoya University, Japan
4 protocols

Mitsutaka Ogawa 4 protocols

Entzu Wan 2 protocols

Ailing Zhang 1 protocol

Shogo Sawaguchi

2 Author merit
Reviewer merit
Editor merit

2 Protocols published

Isolation of Murine Brain and Lung Microvascular Endothelial Cells

Authors: Ailing Zhang, Shogo Sawaguchi, Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 08/20/2018, view: 448, Q&A: 0

This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak et al., 2010; Ruck et al., 2014). We have used the protocol to analyze ...

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Protocol for Notch-ligand Binding Assays Using Dynabeads

Authors: Shogo Sawaguchi, Mitsutaka Ogawa and Tetsuya Okajima, date: 10/20/2017, view: 2444, Q&A: 0

This protocol describes how to measure interaction between Notch receptors and their ligands by cell-based assay using Dynabeads. We have used the protocol to determine binding capacity between Notch1-transfected HEK293T cells and ligand-coated ...

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