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Tetsuya Okajima
Nagoya University, Japan
4 protocols
Mitsutaka Ogawa
4 protocols
Entzu Wan
2 protocols
Ailing Zhang
1 protocol
View all coauthors >
Shogo Sawaguchi
Research focus
Developmental biology
Contributions
2
Author merit
Reviewer merit
Editor merit
2 Protocols published
Isolation of Murine Brain and Lung Microvascular Endothelial Cells
Authors:
Ailing Zhang
,
Shogo Sawaguchi
,
Entzu Wan
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
08/20/2018,
view:
3035,
Q&A:
0
This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak
et al.
, 2010; Ruck
et al.
, 2014). We have used the protocol to analyze ...
More >
Protocol for Notch-ligand Binding Assays Using Dynabeads
Authors:
Shogo Sawaguchi
,
Mitsutaka Ogawa
and
Tetsuya Okajima
,
date:
10/20/2017,
view:
5536,
Q&A:
0
This protocol describes how to measure interaction between Notch receptors and their ligands by cell-based assay using Dynabeads. We have used the protocol to determine binding capacity between Notch1-transfected HEK293T cells and ligand-coated ...
More >
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