Patrick T. Harrison —BIO-PROTOCOL

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David Sanz Physiology, University College Cork, Ireland
1 protocol

Patrick T. Harrison

Department of Physiology, BioSciences Institute, University College Cork, Cork, Ireland

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1 Protocol published

Minigene Assay to Evaluate CRISPR/Cas9-based Excision of Intronic Mutations that Cause Aberrant Splicing in Human Cells

Authors: David J. Sanz and Patrick T. Harrison, date: 06/05/2019, view: 195, Q&A: 0

The construction of Hybrid minigenes provides a robust and simple strategy to study the effects of disease-causing mutations on mRNA splicing when biological material from patient cells is not available. Hybrid minigenes can be used as splicing ...

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