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Coauthors
Ching-Hwa Sung 1 protocol

Wataru Otsu 1 protocol

Kensuke Sakaji 1 protocol

Masaki Saito
  • Department of Molecular Pharmacology, Tohoku University School of Medicine, Japan, Japan,
  • 1 Author merit

Education

PhD, Tohoku University, Japan, 2008

Current position

Assistant Professor, Department of Molecular Pharmacology, Tohoku University School of Medicine, Japan, 

Publications

  1. Zhou, Y., Saito, M., Miyamoto, T., Novak, P., Shevchuk, A. I., Korchev, Y. E., Fukuma, T. and Takahashi, Y. (2018). Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy. Anal Chem 90(4): 2891-2895.
  2. Saito, M., Otsu, W., Hsu, K. S., Chuang, J. Z., Yanagisawa, T., Shieh, V., Kaitsuka, T., Wei, F. Y., Tomizawa, K. and Sung, C. H. (2017). Tctex-1 controls ciliary resorption by regulating branched actin polymerization and endocytosis. EMBO Rep 18(8): 1460-1472.
  3. Arashiki, N., Saito, M., Koshino, I., Kamata, K., Hale, J., Mohandas, N., Manno, S. and Takakuwa, Y. (2016). An Unrecognized Function of Cholesterol: Regulating the Mechanism Controlling Membrane Phospholipid Asymmetry. Biochemistry 55(25): 3504-3513.
  4. Yeh, C., Li, A., Chuang, J. Z., Saito, M., Caceres, A. and Sung, C. H. (2013). IGF-1 activates a cilium-localized noncanonical Gbetagamma signaling pathway that regulates cell-cycle progression. Dev Cell 26(4): 358-368.
  5. Goto, T., Chiba, A., Sukegawa, J., Yanagisawa, T., Saito, M. and Nakahata, N. (2013). Suppression of adenylyl cyclase-mediated cAMP production by plasma membrane associated cytoskeletal protein 4.1G. Cell Signal 25(3): 690-697.
  6. Goto, S., Saito, M., Obara, Y., Moriya, T. and Nakahata, N. (2012). Involvement of lipid rafts in multiple signal transductions mediated by two isoforms of thromboxane A(2) receptor: dependency on receptor isoforms and downstream signaling types. Eur J Pharmacol 693(1-3): 15-24.
  7. Nusuetrong, P., Saito, M., Kikuchi, H., Oshima, Y., Moriya, T. and Nakahata, N. (2012). Apoptotic effects of satratoxin H is mediated through DNA double-stranded break in PC12 cells. J Toxicol Sci 37(4): 803-812.
  8. Li, A., Saito, M., Chuang, J. Z., Tseng, Y. Y., Dedesma, C., Tomizawa, K., Kaitsuka, T. and Sung, C. H. (2011). Ciliary transition zone activation of phosphorylated Tctex-1 controls ciliary resorption, S-phase entry and fate of neural progenitors. Nat Cell Biol 13(4): 402-411.
  9. Conde, C., Arias, C., Robin, M., Li, A., Saito, M., Chuang, J. Z., Nairn, A. C., Sung, C. H. and Caceres, A. (2010). Evidence for the involvement of Lfc and Tctex-1 in axon formation. J Neurosci 30(19): 6793-6800.
  10. Saito, M., Tanaka, H., Sasaki, M., Kurose, H. and Nakahata, N. (2010). Involvement of aquaporin in thromboxane A2 receptor-mediated, G 12/13/RhoA/NHE-sensitive cell swelling in 1321N1 human astrocytoma cells. Cell Signal 22(1): 41-46.
  11. Uchiyama, K., Saito, M., Sasaki, M., Obara, Y., Higashiyama, S. and Nakahata, N. (2009). Thromboxane A2 receptor-mediated epidermal growth factor receptor transactivation: involvement of PKC-delta and PKC-epsilon in the shedding of epidermal growth factor receptor ligands. Eur J Pharm Sci 38(5): 504-511.
  12. Nasu, Y., Iwashita, M., Saito, M., Fushiya, S. and Nakahata, N. (2009). Inhibitory effects of Atractylodis lanceae rhizoma and Poria on collagen- or thromboxane A2-induced aggregation in rabbit platelets. Biol Pharm Bull 32(5): 856-860.
  13. Honma, S., Saito, M., Kikuchi, H., Saito, Y., Oshima, Y., Nakahata, N. and Yoshida, M. (2009). A reduction of epidermal growth factor receptor is involved in brefelamide-induced inhibition of phosphorylation of ERK in human astrocytoma cells. Eur J Pharmacol 616(1-3): 38-42.
  14. Saito, M., Shinohara, Y., Sasaki, H., Netsu, Y., Yoshida, M. and Nakahata, N. (2008). Type 1 angiotensin receptor (AT1-R)-mediated decrease in type 2 angiotensin receptor mRNA level is dependent on Gq and extracellular signal-regulated kinase 1//2 in AT1-R-transfected PC12 cells. J Neuroendocrinol 20(3): 299-308.
  15. Iwashita, M., Oka, N., Ohkubo, S., Saito, M. and Nakahata, N. (2007). Piperlongumine, a constituent of Piper longum L., inhibits rabbit platelet aggregation as a thromboxane A(2) receptor antagonist. Eur J Pharmacol 570(1-3): 38-42.
  16. Iwashita, M., Saito, M., Yamaguchi, Y., Takagaki, R. and Nakahata, N. (2007). Inhibitory effect of ethanol extract of Piper longum L. on rabbit platelet aggregation through antagonizing thromboxane A2 receptor. Biol Pharm Bull 30(7): 1221-1225.
  17. Saito, M., Hori, M., Obara, Y., Ohizumi, Y., Ohkubo, S. and Nakahata, N. (2006). Neurotrophic factor production in human astrocytoma cells by 2,5,6-tribromogramine via activation of epsilon isoform of protein kinase C. Eur J Pharm Sci 28(4): 263-271.
  18. Kikuchi, H., Saito, Y., Sekiya, J., Okano, Y., Saito, M., Nakahata, N., Kubohara, Y. and Oshima, Y. (2005). Isolation and synthesis of a new aromatic compound, brefelamide, from dictyostelium cellular slime molds and its inhibitory effect on the proliferation of astrocytoma cells. J Org Chem 70(22): 8854-8858.
  19. Saito, M., Sugai, M., Katsushima, Y., Yanagisawa, T., Sukegawa, J. and Nakahata, N. (2005). Increase in cell-surface localization of parathyroid hormone receptor by cytoskeletal protein 4.1G. Biochem J 392(Pt 1): 75-81.
  20. Sugai, M., Saito, M., Sukegawa, I., Katsushima, Y., Kinouchi, Y., Nakahata, N., Shimosegawa, T., Yanagisawa, T. and Sukegawa, J. (2003). PTH/PTH-related protein receptor interacts directly with Tctex-1 through its COOH terminus. Biochem Biophys Res Commun 311(1): 24-31.
1 Protocol published
Ciliary Assembly/Disassembly Assay in Non-transformed Cell Lines
Authors:  Masaki Saito, Kensuke Sakaji, Wataru Otsu and Ching-Hwa Sung, date: 03/20/2018, view: 1494, Q&A: 0
The primary cilium is a non-motile sensory organelle whose assembly and disassembly are closely associated with cell cycle progression. The primary cilium is elongated from the basal body in quiescent cells and is resorbed as the cells re-enter the ...