Hello,

I was confused because of TO1.
In the paper from the same authors (2014, RNA Mango Aptamer-Fluorophore: A Bright, High-Affinity Complex for RNA Labeling and Tracking)
it is mentioned, that TO1 binds nonspecifically to DNA and RNA that lead to high background, to avoid this TO1-acetate was synthesized. It 2017, in "Ribonucleoprotein purification and characterization using RNA Mango" also was used TO1-acetate. Now, in this protocol, commercial TO1-PEG3-Desthiobiotin is used. Is this commercial TO1-PEG3-Desthiobiotin has acetate, or it does not have high background because of other reasons?

Looking forward for your answer!
I am really curious if I can use this system for my experiment!

Best wishes,
Nataliia. 8/12/2020 5:55:31 AM Reply