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Coauthors
L. Sibley
Washington University in St Louis, USA
2 protocols
Kevin Brown
Molecular Microbiology, Washington University in St Louis, USA
2 protocols
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Shaojun Long
Molecular Microbiology, Washington University in St Louis, USA
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2 Protocols published
CRISPR-mediated Tagging with BirA Allows Proximity Labeling in
Toxoplasma gondii
Authors:
Shaojun Long
,
Kevin M. Brown
and
L. David Sibley
,
date:
03/20/2018,
view:
1180,
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0
Defining protein interaction networks can provide key insights into how protein complexes govern complex biological problems. Here we define a method for proximity based labeling using permissive biotin ligase to define protein networks in the ...
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Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in
Toxoplasma gondii
Authors:
Kevin M. Brown
,
Shaojun Long
and
L. David Sibley
,
date:
02/20/2018,
view:
1804,
Q&A:
0
Toxoplasma gondii
is a member of the deadly phylum of protozoan parasites called Apicomplexa. As a model apicomplexan, there is a great wealth of information regarding
T. gondii
’s 8,000+ protein coding genes including sequence ...
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