Gregory Finnigan Kansas State University
2 protocols

Rachael Marie Giersch
  • Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
  • 1 Author merit
1 Protocol published
Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
Authors:  Rachael M. Giersch and Gregory C. Finnigan, date: 09/20/2017, view: 3542, Q&A: 0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...