Nigel Temperton Vial Pseudotype Unit, Medway School of Pharmacy, University of Kent, United Kingdom, United Kingdom,
2 protocols

Fabrizio Biuso 1 protocol

Eleonora Molesti 1 protocol
Jeremy Welsch University of Michigan, Medical School
3 protocols

Longping Tse University of North Carolina at Chapel Hill
25 protocols

Masfique Mehedi University of North Dakota
2 protocols

Vikas Duhan Institute for Immunology
1 protocol

Reviewer
George William Carnell
  • Post-Doc, University of Cambridge
Research focus
  • Immunology
  • 2 Author merit

Education

PhD, University of Kent, 2018

2 Protocols published
A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
Authors:  Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 2358, Q&A: 0
This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes
Authors:  George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 6036, Q&A: 0
The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...
3 Protocols reviewed
Production of Quantum Dots-containing Influenza Virus Particles for Studying Viral Uncoating Processes
Authors:  Chong Qin, Xiaowei Zhang and Zongqiang Cui, date: 09/20/2019, view: 343, Q&A: 0
The genome of influenza A virus (IAV) comprises eight pinlike genomic segments called vRNPs enclosed in viral capsid. During infection, uncoating is the key step for viral replication and represents an antiviral therapeutic target, but it is ...
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Sendai Virus Propagation Using Chicken Eggs
Authors:  Narihito Tatsumoto, Moshe Arditi and Michifumi Yamashita, date: 09/20/2018, view: 2093, Q&A: 0
Sendai virus is a member of the family Paramyxoviridae, and an enveloped virus with a negative-stranded RNA genome. Sendai virus is not pathogenic to humans, but for mice and can cause pneumonia in mice. Easy and efficient techniques for ...
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