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Coauthors
Nigel Temperton Vial Pseudotype Unit, Medway School of Pharmacy, University of Kent, United Kingdom, United Kingdom,
2 protocols

Fabrizio Biuso 1 protocol

Eleonora Molesti 1 protocol
Coreviewers
Longping Tse University of North Carolina at Chapel Hill
17 protocols

Masfique Mehedi University of North Dakota
2 protocols

Vikas Duhan Institute for Immunology
1 protocol

Reviewer
George Carnell
  • University of Kent, United Kingdom
  • 2 Author merit
2 Protocols published
A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase
Authors:  Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 335, Q&A: 0
This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...
An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes
Authors:  George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 3255, Q&A: 0
The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...
2 Protocols reviewed
Sendai Virus Propagation Using Chicken Eggs
Authors:  Narihito Tatsumoto, Moshe Arditi and Michifumi Yamashita, date: 09/20/2018, view: 34, Q&A: 0
Sendai virus is a member of the family Paramyxoviridae, and an enveloped virus with a negative-stranded RNA genome. Sendai virus is not pathogenic to humans, but for mice and can cause pneumonia in mice. Easy and efficient techniques for ...
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Nab Escaping AAV Mutants Isolated from Mouse Muscles
Authors:  Zheng Chai, R. Jude Samulski and Chengwen Li, date: 05/05/2018, view: 951, Q&A: 0
Neutralizing antibodies (Nabs) are a major challenge in clinical trials of adeno-associated virus (AAV) vector gene therapy, because Nabs are able to inhibit AAV transduction in patients. We have successfully isolated several novel Nab-escaped AAV ...
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