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Alejandro Buschiazzo Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Felipe Trajtenberg Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Nicole Larrieux Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Juan Imelio Laboratory of Molecular and Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Ariel Edgardo Mechaly
  • Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
  • 1 Author merit

Education

PhD in Biochemistry and Molecular Biology, University of the Basque Country, Spain, 2009

Current position

Postdoc, Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay

Publications

  1. Mechaly, A. E., Soto Diaz, S., Sassoon, N., Buschiazzo, A., Betton, J. M. and Alzari, P. M. (2017). Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase. Structure 25(6): 939-944 e933.
  2. San Martin, F., Mechaly, A. E., Larrieux, N., Wunder, E. A., Jr., Ko, A. I., Picardeau, M., Trajtenberg, F. and Buschiazzo, A. (2017). Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis. Acta Crystallogr F Struct Biol Commun 73(Pt 3): 123-129.
  3. Trajtenberg, F., Imelio, J. A., Machado, M. R., Larrieux, N., Marti, M. A., Obal, G., Mechaly, A. E. and Buschiazzo, A. (2016). Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action. Elife 5.
  4. Fouts, D. E., Matthias, M. A., Adhikarla, H., Adler, B., Amorim-Santos, L., Berg, D. E., Bulach, D., Buschiazzo, A., Chang, Y. F., Galloway, R. L., Haake, D. A., Haft, D. H., Hartskeerl, R., Ko, A. I., Levett, P. N., Matsunaga, J., Mechaly, A. E., Monk, J. M., Nascimento, A. L., Nelson, K. E., Palsson, B., Peacock, S. J., Picardeau, M., Ricaldi, J. N., Thaipandungpanit, J., Wunder, E. A., Jr., Yang, X. F., Zhang, J. J. and Vinetz, J. M. (2016). What Makes a Bacterial Species Pathogenic?:Comparative Genomic Analysis of the Genus Leptospira. PLoS Negl Trop Dis 10(2): e0004403.
  5. East, A., Mechaly, A. E., Huysmans, G. H., Bernarde, C., Tello-Manigne, D., Nadeau, N., Pugsley, A. P., Buschiazzo, A., Alzari, P. M., Bond, P. J. and Francetic, O. (2016). Structural Basis of Pullulanase Membrane Binding and Secretion Revealed by X-Ray Crystallography, Molecular Dynamics and Biochemical Analysis. Structure 24(1): 92-104.
  6. Trajtenberg, F., Albanesi, D., Ruétalo, N., Botti, H., Mechaly, A. E., Nieves, M., Aguilar, P. S., Cybulski, L., Larrieux, N., de Mendoza, D. and Buschiazzo, A. (2014). Allosteric Activation of Bacterial Response Regulators: the Role of the Cognate Histidine Kinase Beyond Phosphorylation. mBio 5(6): e02105-14.
  7. Correa, A., Pacheco, S., Mechaly, A. E., Obal, G., Behar, G., Mouratou, B., Oppezzo, P., Alzari, P. M. and Pecorari, F. (2014). Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins). PLoS One 9(5): e97438.
  8. Mechaly, A. E., Sassoon, N., Betton, J. M. and Alzari, P. M. (2014). Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. PLoS Biol 12(1): e1001776.
  9. Mechaly, A. E., Haouz, A., Miras, I., Barilone, N., Weber, P., Shepard, W., Alzari, P. M. and Bellinzoni, M. (2012). Conformational changes upon ligand binding in the essential class II fumarase Rv1098c from Mycobacterium tuberculosis. FEBS Lett 586(11): 1606-1611.
  10. Mechaly, A. E., Bellomio, A., Morante, K., Agirre, J., Gil-Carton, D., Valle, M., Gonzalez-Manas, J. M. and Guerin, D. M. (2012). Pores of the toxin FraC assemble into 2D hexagonal clusters in both crystal structures and model membranes. J Struct Biol 180(2): 312-317.
  11. Mechaly, A. E., Bellomio, A., Gil-Carton, D., Morante, K., Valle, M., Gonzalez-Manas, J. M. and Guerin, D. M. (2011). Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins. Structure 19(2): 181-191.
  12. Mechaly, A. E., Bellomio, A., Morante, K., Gonzalez-Manas, J. M. and Guerin, D. M. (2009). Crystallization and preliminary crystallographic analysis of fragaceatoxin C, a pore-forming toxin from the sea anemone Actinia fragacea. Acta Crystallogr Sect F Struct Biol Cryst Commun 65(Pt 4): 357-360.
  13. Agirre, J., Mechaly, A., Cabo-Bilbao, A. and Guerin, D. M. (2008). A plate holder for non-destructive testing of mesophase crystallization assays. Eur Biophys J 37(6): 871-877.
  14. Cabo-Bilbao, A., Spinelli, S., Sot, B., Agirre, J., Mechaly, A. E., Muga, A. and Guerin, D. M. (2006). Crystal structure of the temperature-sensitive and allosteric-defective chaperonin GroELE461K. J Struct Biol 155(3): 482-492.
1 Protocol published
Snapshots of the Signaling Complex DesK:DesR in Different Functional States Using Rational Mutagenesis and X-ray Crystallography
We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in E. coli ...