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Alejandro Buschiazzo
Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol
Felipe Trajtenberg
Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol
Ariel Mechaly
Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol
Nicole Larrieux
Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol
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Juan Andres Imelio
Laboratory of Molecular and Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
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1 Protocol published
Snapshots of the Signaling Complex DesK:DesR in Different Functional States Using Rational Mutagenesis and X-ray Crystallography
Authors:
Juan Andres Imelio
,
Nicole Larrieux
,
Ariel Edgardo Mechaly
,
Felipe Trajtenberg
and
Alejandro Buschiazzo
,
date:
08/20/2017,
view:
3680,
Q&A:
0
We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in
E. coli
...
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