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Eugene I Shakhnovich
Department of Chemistry and Chemical Biology, Harvard University, USA
1 protocol
Shimon Bershtein
Department of Life Sciences, Ben-Gurion University of the Negev, Israel
1 protocol
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Sanchari Bhattacharyya
Department of Chemistry and Chemical Biology, Harvard University, USA
Research focus
Microbiology
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1 Protocol published
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Authors:
Sanchari Bhattacharyya
,
Shimon Bershtein
and
Eugene I Shakhnovich
,
date:
07/20/2017,
view:
5055,
Q&A:
0
This protocol is used to assay the effect of protein over-expression on fitness of
E. coli
. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...
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