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Xu Zheng
Whitehead Institute for Biomedical Research, USA
1 protocol
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David Pincus
Whitehead Institute for Biomedical Research, USA
Research focus
Microbiology
Contributions
1
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Education
Ph.D, UCSF, USA, 2000
Current position
Whitehead Fellow, n/a, Whitehead Institute, USA
1 Protocol published
Serial Immunoprecipitation of 3xFLAG/V5-tagged Yeast Proteins to Identify Specific Interactions with Chaperone Proteins
Authors:
Xu Zheng
and
David Pincus
,
date:
06/20/2017,
view:
5546,
Q&A:
0
This method was generated to isolate high affinity protein complexes from yeast lysate by performing serial affinity purification of doubly tagged 3xFLAG/V5 proteins. First, the bait protein of interest is immunoprecipitated by anti-FLAG-conjugated ...
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