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David Millar
1 protocol
Raymond Pauszek III
Department of Integrative Structural & Computational Biology, the Scripps Research Institute, USA
1 protocol
Jeffrey Liu
Department of Integrative Structural & Computational Biology, the Scripps Research Institute, USA
1 protocol
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Rajan Lamichhane
Department of Integrative Structural & Computational Biology, the Scripps Research Institute, USA
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1 Protocol published
Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor
Authors:
Rajan Lamichhane
,
Jeffrey J. Liu
,
Raymond F. Pauszek III
and
David P. Millar
,
date:
06/20/2017,
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2425,
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Activation of G protein-coupled receptors (GPCRs) by agonist ligands is mediated by a transition from an inactive to active receptor conformation. We describe a novel single-molecule assay that monitors activation-linked conformational transitions ...
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