Search
Coauthors
Ryan Schaub Medical Microbiology & Immunology, University of Wisconsin-Madison, USA
1 protocol

Joseph P Dillard
  • Medical Microbiology & Immunology, University of Wisconsin-Madison, USA
  • 1 Author merit

Education

Ph.D., University of Alabama at Birmingham, USA, 1994

Current position

Professor, Medical Microbiolgy and Immunology, University of Wisconsin-Madison, USA

Publications

  1. Knilans, K. J., Hackett, K. T., Anderson, J. E., Weng, C., Dillard, J. P. and Duncan, J. A. (2017). Neisseria gonorrhoeae Lytic Transglycosylases LtgA and LtgD Reduce Host Innate Immune Signaling through TLR2 and NOD2. ACS Infect Dis.
  2. Ragland, S. A., Schaub, R. E., Hackett, K. T., Dillard, J. P. and Criss, A. K. (2017). Two lytic transglycosylases in Neisseria gonorrhoeae impart resistance to killing by lysozyme and human neutrophils. Cell Microbiol 19(3).
  3. Schaub, R. E., Chan, Y. A., Lee, M., Hesek, D., Mobashery, S. and Dillard, J. P. (2016). Lytic transglycosylases LtgA and LtgD perform distinct roles in remodeling, recycling and releasing peptidoglycan in Neisseria gonorrhoeae. Mol Microbiol 102(5): 865-881.
  4. Harrison, O. B., Clemence, M., Dillard, J. P., Tang, C. M., Trees, D., Grad, Y. H. and Maiden, M. C. (2016). Genomic analyses of Neisseria gonorrhoeae reveal an association of the gonococcal genetic island with antimicrobial resistance. J Infect 73(6): 578-587.
  5. Chan, J. M. and Dillard, J. P. (2016). Neisseria gonorrhoeae Crippled Its Peptidoglycan Fragment Permease To Facilitate Toxic Peptidoglycan Monomer Release. J Bacteriol 198(21): 3029-3040.
  6. Lenz, J. D., Stohl, E. A., Robertson, R. M., Hackett, K. T., Fisher, K., Xiong, K., Lee, M., Hesek, D., Mobashery, S., Seifert, H. S., Davies, C. and Dillard, J. P. (2016). Amidase Activity of AmiC Controls Cell Separation and Stem Peptide Release and Is Enhanced by NlpD in Neisseria gonorrhoeae. J Biol Chem 291(20): 10916-10933.
  7. Schaub, R. E., Lenz, J. D. and Dillard, J. P. (2016). Analysis of Peptidoglycan Fragment Release. Methods Mol Biol 1440: 185-200.
  8. Andrade, W. A., Agarwal, S., Mo, S., Shaffer, S. A., Dillard, J. P., Schmidt, T., Hornung, V., Fitzgerald, K. A., Kurt-Jones, E. A. and Golenbock, D. T. (2016). Type I Interferon Induction by Neisseria gonorrhoeae: Dual Requirement of Cyclic GMP-AMP Synthase and Toll-like Receptor 4. Cell Rep 15(11): 2438-2448.
  9. Bhoopalan, S. V., Piekarowicz, A., Lenz, J. D., Dillard, J. P. and Stein, D. C. (2016). nagZ Triggers Gonococcal Biofilm Disassembly. Sci Rep 6: 22372.
  10. Stohl, E. A., Lenz, J. D., Dillard, J. P. and Seifert, H. S. (2015). The gonococcal NlpD protein facilitates cell separation by activating peptidoglycan cleavage by AmiC. J Bacteriol 198: 615-22.
  11. Krasity, B. C., Troll, J. V., Lehnert, E. M., Hackett, K. T., Dillard, J. P., Apicella, M. A., Goldman, W. E., Weiss, J. P. and McFall-Ngai, M. J. (2015). Structural and functional features of a developmentally regulated lipopolysaccharide-binding protein. MBio 6(5): e01193-01115.
  12. Ramsey, M. E., Bender, T., Klimowicz, A. K., Hackett, K. T., Yamamoto, A., Jolicoeur, A., Callaghan, M. M., Wassarman, K. M., van der Does, C. and Dillard, J. P. (2015). Targeted mutagenesis of intergenic regions in the Neisseria gonorrhoeae gonococcal genetic island reveals multiple regulatory mechanisms controlling type IV secretion. Mol Microbiol 97(6): 1168-1185.
  13. Dillard, J. P. (2014). Peptidoglycan metabolism and fragment production. In: Davies, J. K. and Kahler, C. (Eds.). Pathogenic Neisseria: Genomics, Molecular Biology and Disease Intervention. Horizon Press.
  14. Pachulec, E., Siewering, K., Bender, T., Heller, E. M., Salgado-Pabon, W., Schmoller, S. K., Woodhams, K. L., Dillard, J. P. and van der Does, C. (2014). Functional analysis of the Gonococcal Genetic Island of Neisseria gonorrhoeae. PLoS One 9(10): e109613.
  15. Ramsey, M. E., Hackett, K. T., Bender, T., Kotha, C., van der Does, C. and Dillard, J. P. (2014). TraK and TraB are conserved outer membrane proteins of the Neisseria gonorrhoeae Type IV secretion system and are expressed at low levels in wild-type cells. J Bacteriol 196(16): 2954-2968.
  16. Woodhams, K. L., Chan, J. M., Lenz, J. D., Hackett, K. T. and Dillard, J. P. (2013). Peptidoglycan fragment release from Neisseria meningitidis. Infect Immun 81(9): 3490-3498.
  17. Kohler, P. L., Chan, Y. A., Hackett, K. T., Turner, N., Hamilton, H. L., Cloud-Hansen, K. A. and Dillard, J. P. (2013). Mating pair formation homologue TraG is a variable membrane protein essential for contact-independent type IV secretion of chromosomal DNA by Neisseria gonorrhoeae. J Bacteriol 195(8): 1666-1679.
  18. Horner, J., Massey, E. W., Riski, J. E., Lathrop, D. L. and Chase, K. N. (1988). Aspiration following stroke: clinical correlates and outcome. Neurology 38(9): 1359-1362.
  19. Jain, S., Zweig, M., Peeters, E., Siewering, K., Hackett, K. T., Dillard, J. P. and van der Does, C. (2012). Characterization of the single stranded DNA binding protein SsbB encoded in the Gonoccocal Genetic Island. PLoS One 7(4): e35285.
  20. Woodhams, K. L., Benet, Z. L., Blonsky, S. E., Hackett, K. T. and Dillard, J. P. (2012). Prevalence and detailed mapping of the gonococcal genetic island in Neisseria meningitidis. J Bacteriol 194(9): 2275-2285.
  21. Stohl*, E. A., Chan*, Y. A. (co-first author), Hackett, K. T., Kohler, P. L., Dillard, J. P. and Seifert, H. S. (2012). The Neisseria gonorrhoeae virulence factor NG1686 is a bifunctional M23B family metallopeptidase that influences resistance to hydrogen peroxide and colony morphology. J Biol Chem 287: 11222-11233.
  22. Ramsey, M. E., Hackett, K. T., Kotha, C. and Dillard, J. P. (2012). New complementation constructs for inducible and constitutive gene expression in Neisseria gonorrhoeae and Neisseria meningitidis. Appl Environ Microbiol 78(9): 3068-3078.
  23. Ramsey, M. E., Woodhams, K. L. and Dillard, J. P. (2011). The Gonococcal Genetic Island and Type IV Secretion in the Pathogenic Neisseria. Front Microbiol 2: 61.
  24. Dominguez, N. M., Hackett, K. T. and Dillard, J. P. (2011). XerCD-mediated site-specific recombination leads to loss of the 57-kilobase gonococcal genetic island. J Bacteriol 193(2): 377-388.
  25. Dillard, J. P. (2011). Genetic Manipulation of Neisseria gonorrhoeae. Curr Protoc Microbiol Chapter 4: Unit4A 2.
1 Protocol published
Digestion of Peptidoglycan and Analysis of Soluble Fragments
Authors:  Ryan E. Schaub and Joseph P. Dillard, date: 08/05/2017, view: 2027, Q&A: 0
Peptidoglycan (murein) is a vital component of the cell wall of nearly all bacteria, composed of sugars linked by short peptides. This protocol describes the purification of macromolecular peptidoglycan from cultured bacteria and the analysis of ...