Mario de Bono 1 protocol

Eisuke Itakura Department of Biology, Graduate School of Science, Chiba University, Japan, Japan,
1 protocol

Changchun Chen
  • 1 Author merit
1 Protocol published
Purification of FLAG-tagged Secreted Proteins from Mammalian Cells
Authors:  Eisuke Itakura, Changchun Chen and Mario de Bono, date: 08/05/2017, view: 3598, Q&A: 0
This protocol describes a method for purifying glycosylated FLAG-tagged secreted proteins with disulfide bonds from mammalian cells. The purified products can be used for various applications, such as ligand binding assays.