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Rubul Mout
Institute for Protein Design, University of Washington
2 protocols
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Vincent M Rotello
University of Massachusetts, USA
Research focus
Cell biology
Contributions
2
Author merit
Reviewer merit
Editor merit
2 Protocols published
A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles
Authors:
Rubul Mout
and
Vincent M. Rotello
,
date:
12/20/2017,
view:
5292,
Q&A:
0
In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using ...
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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:
Rubul Mout
and
Vincent M. Rotello
,
date:
10/20/2017,
view:
8790,
Q&A:
1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
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Ali Salimi
Iran university of medical science
I have not been work crispr cas method for any work yet.
11/30/2018 2:53:02 AM
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