Vincent M Rotello —BIO-PROTOCOL

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Rubul Mout University of Washington , USA
2 protocols

Vincent M Rotello

University of Massachusetts, USA

2 Author merit
Reviewer merit
Editor merit

2 Protocols published

A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles

Authors: Rubul Mout and Vincent M. Rotello, date: 12/20/2017, view: 1940, Q&A: 0

In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using ...

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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles

Authors: Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 3766, Q&A: 0

In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...

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