Ewen Mullins Teagasc, Dept. of Crop Science, Ireland, Ireland,
1 protocol

Fiona Doohan Earth Institute and School of Biology and Environmental Sciences, University College Dublin, Ireland, Ireland,
4 protocols
Amey Redkar Department of Genetics, University of Cordoba
21 protocols

Dheeraj Singh Rathore
  • Crop Improvement Researcher, Teagasc
Research focus
  • Plant science
  • Genome editing, Genetic engineering and Crop Improvement, microbiology, Plant-microbe interaction, Metagenomics, Horticultural crops, Plant pathology, Microbe-microbe interaction, Bio-control
  • 1 Author merit


PhD, University College Dublin, 2017

Lab information

Dr. Ewen Mullins' Lab in Teagasc, Crop Science Department, Biotechnology unit, Oak Park, Carlow R93 XE12, Ireland


Profiling antibiotic resistance and electrotransformation potential of Ensifer adhaerens OV14; a non-Agrobacterium species capable of efficient rates of plant transformation. DS Rathore, MA Lopez-Vernaza, F Doohan, DO Connell, A Lloyd, Mulins E (2015). FEMS microbiology letters 362 (17).

DS Rathore, F Doohan, E Mullins (2016). Capability of the plant-associated bacterium, Ensifer adhaerens strain OV14, to genetically transform its original host Brassica napus. Plant Cell, Tissue and Organ Culture (PCTOC) 127 (1), 85-94.

Rathore, D. S., Doohan, F. M. and Mullins (2017). Ensifer-mediated Arabidopsis thaliana Root Transformation (E-ART): A Protocol to Analyse the Factors that Support Ensifer-mediated Transformation (EMT) of Plant Cells. Bio-Protocol 7 (19), e2564.

DS Rathore, A Govindan, F Doohan, E Mullins (2018). Enhancing the utility of Ensifer-mediated transformation (EMT) technology. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 54, S104-S105

E Mullins, DS Rathore, F Doohan, E Zuniga (2018). Ensifer Mediated Transformation (EMT), a novel engineering platform for crop genomes. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 54, S98-S98

DS Rathore, E Mullins (2018). Alternative Non‐Agrobacterium Based Methods for Plant Transformation. Annual Plant Reviews online, 891-908.

DS Rathore, E Zuniga-Soto, E Mullins (2018). Ensifer-Mediated Transformation (EMT) of Rice (Monocot) and Oilseed Rape (Dicot). Transgenic Plants, 37-48
1 Protocol published
Ensifer-mediated Arabidopsis thaliana Root Transformation (E-ART): A Protocol to Analyse the Factors that Support Ensifer-mediated Transformation (EMT) of Plant Cells
Authors:  Dheeraj Singh Rathore, Fiona M. Doohan and Ewen Mullins, date: 10/05/2017, view: 6279, Q&A: 0
Ensifer adhaerens OV14, a soil borne alpha-proteobacteria of the Rhizobiaceae family, fortifies the novel plant transformation technology platform termed ‘Ensifer-mediated transformation’ (EMT). EMT can stably transform both ...
2 Protocols reviewed
Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
Authors:  Susanna Keriö, Eeva Terhonen and Jared M. LeBoldus, date: 06/05/2020, view: 996, Q&A: 0
We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples ...
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Pneumatic Method to Measure Plant Xylem Embolism
Authors:  Paulo R. L. Bittencourt, Luciano Pereira and Rafael S. Oliveira, date: 10/20/2018, view: 2835, Q&A: 0
Embolism, the formation of air bubbles in the plant water transport system, has a major impact on plant water relations. Embolism formation in the water transport system of plants disrupts plant water transport capacity, impairing plant functioning ...
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