Minsoo Kim —BIO-PROTOCOL

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Elizabeth Vierling Department of Biochemistry & Molecular Biology, University of Massachusetts Amherst, USA
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Eman Basha 1 protocol

Fionn McLoughlin 1 protocol

Minsoo Kim

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1 Protocol published

Assessing Plant Tolerance to Acute Heat Stress

Authors: Minsoo Kim, Fionn McLoughlin, Eman Basha and Elizabeth Vierling, date: 07/20/2017, view: 3061, Q&A: 1

It is well-established that plants are able to acclimate to temperatures above or below the optimal temperature for their growth. Here, we provide protocols for assays that can be used quantitatively or qualitatively to assess the relative ability ...

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Johan Zicola

Max Planck Institute for Plant Breeding Research

I was wondering what are the pros and cons to use Parafilm to seal the Petri dishes, compared to gaz-permeable tapes (e.g. Leukopor). I noticed plants are 'sweating' when plates are sealed with Parafilm during heat-stress as there are no gaz exchange and I may think that it has an impact on the heat-stress resistance outcome (I did not do a proper comparison though). However, I could find papers using either Parafilm or Leukopor (often not mentioned at all in M&Ms actually) but I could not really find a serious comparison between the 2 methods. Could you share your experience on the subject?
Thanks!

10/12/2018 2:16:17 AM Reply

Minsoo Kim

Hi Johan,
I actually have had the same question, but I haven't tried to compare the effects of using parafilm vs. gas-permeable tapes.
My guess is plants might be more tolerant if there was gas
exchange because of less humidity, higher transpiration rate and
cooling. If you get to try, please share your results with us.
Thanks!
Minsoo

2018-10-12 07:30:44