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Shuji Takada
1 protocol
Miho Terao
Department of Systems BioMedicine, National Research Institute for Child Health and Development, Japan
1 protocol
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Satoshi Hara
Department of Systems BioMedicine, National Research Institute for Child Health and Development, Japan
Research focus
Molecular biology
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1 Protocol published
A Protocol for Production of Mutant Mice Using Chemically Synthesized crRNA/tracrRNA with Cas9 Nickase and FokI-dCas9
Authors:
Satoshi Hara
,
Miho Terao
and
Shuji Takada
,
date:
06/05/2017,
view:
7498,
Q&A:
0
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is the most widely used genome editing tool. A common CRISPR/Cas9 system consists of two components: a single-guide RNA (sgRNA) and ...
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