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Coauthors
Chien-Hong Chen Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
1 protocol

Chin-kai Chuang Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
1 protocol

Ching-Fu Tu
  • Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Taiwan
  • 1 Author merit

Education

Ph.D in Animal Science, Department of Animal Science, National Taiwan University, Taiwan ROC., 1994

Current position

Principal Researcher, Division of Animal Technology, Animal Technology Laboratories, Agriculture Technology Research Institute

Publications (since 1995)

  1. Chuang, C. K., Chen, C. H., Huang, C. L., Su, Y. H., Peng, S. H., Lin, T. Y., Tai, H. C., Yang, T. S. and Tu, C. F. (2016). Generation of GGTA1 Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors. Anim Biotechnol: 1-8.
  2. Chuang, C. K., Chen, C. H., Su, Y. S., Peng, S. H., Lin, T. Y., Huang, C. L., Yang, T. S. and Tu, C. F. (2016). Generation of GGTA1 knockout pigs by using direct pronuclear microinjection with TALEN plasmid DNA vectors. J Chin Soc Anim Sci 45(3): 223-240.
  3. Yen, C. H., Yang, T. S., Lin, Y. S., Lee, M. H., Yu, K. C., Huang, C. L., Hsieh, H. H. and Tu, C. F. (2016). A galactopoiesis accordant yield of functional recombinant human factor IX from homozygous transgenic pigs requires a large amount of vitamin K supplementation. Transgenic Res 25(4): 545-551.
  4. Liu, P. C., Chen, C. A., Chen, C. M., Yen, C. H., Lee, M. H., Chuang, C. K., Tu, C. F. and Su, B. L. (2016). Application of xenogeneic anti-canine distemper virus antibodies in treatment of canine distemper puppies. J Small Anim Pract 57(11): 626-630.
  5. Yen, C. H., Lin, Y. S. and Tu, C. F. (2015). A novel method for separation of caseins from milk by phosphates precipitation. Prep Biochem Biotechnol 45(1): 18-32.
  6. Siriboon, C., Lin, Y. H., Kere, M., Chen, C. D., Chen, L. R., Chen, C. H., Tu, C. F., Lo, N. W. and Ju, J. C. (2015). Putative porcine embryonic stem cell lines derived from aggregated four-celled cloned embryos produced by oocyte bisection cloning. PLoS One 10(2): e0118165.
  7. Su, Y. H., Lin, T. Y., Huang, C. L., Tu, C. F. and Chuang, C. K. (2015). Construction of a CRISPR-Cas9 System for Pig Genome Targeting. Anim Biotechnol 26(4): 279-288.
  8. Lee, T. J., Chen, Y. H., Chen, C. J., Wang, S. H., Liu, H. T., Tu, C. F. and Huang, S. Y. (2015). Comparison of protein expression in the mammary gland and milk from hirudin transgenic and non-transgenic sows using a proteomic approach. J Chin Soc Anim Sci 44(1): 35-62.
  9. Siriboon, C., Tu, C. F., Kere, M., Liu, M. S., Chang, H. J., Ho, L. L., Tai, M. E., Fang, W. D., Lo, N. W., Tseng, J. K. and Ju, J. C. (2014). Production of viable cloned miniature pigs by aggregation of handmade cloned embryos at the 4-cell stage. Reprod Fertil Dev 26(3): 395-406.
  10. Lee, M. H., Lin, Y. S., Tu, C. F. and Yen, C. H. (2014). Recombinant human factor IX produced from transgenic porcine milk. Biomed Res Int 2014: 315375.
  11. Sun, Y. L., Yen, C. H. and Tu, C. F. (2014). Visual detection of canine parvovirus based on loop-mediated isothermal amplification combined with enzyme-linked immunosorbent assay and with lateral flow dipstick. J Vet Med Sci 76(4): 509-516.
  12. Chen, C. H., Jiang, B. H., Huang, S. Y., Yang, T. S., Lee, K. H., Tu, C. F. and Wu, S. C. (2013). Genetic polymorphisms, growth performance, blood profiles, and reproductive characteristics in phenotypically normal Landrace boars produced by somatic cell nuclear transfer. Theriogenology 80(9): 1088-1096.
  13. Lee, K. H., Sun, J. C., Chuang, C. K., Guo, S. F., Tu, C. F. and Ju, J. C. (2013). An efficient and mass reproducible method for vitrifying mouse embryos on a paper in cryotubes. Cryobiology 66(3): 311-317.
  14. Yu, C. Z., Liu, H. T., Guo, S. F., Jiang, B. H., Yang, T. S., Yen, C. H., Hsieh, H. H. and Tu, C. F. (2013). Effect of vitamin K supplementation on blood characteristics and reproduction performance of human factor IX homozygotic transgenic sows. J Chin Soc Anim Sci 42(2): 97-111.
  15. Lee, K. H., Chuang, C. K., Guo, S. F. and Tu, C. F. (2012). Simple and efficient derivation of mouse embryonic stem cell lines using differentiation inhibitors or proliferation stimulators. Stem Cells Dev 21(3): 373-383.
  16. Huang, S. Y., Lo, L. L., Chung, M. T., Lie, H. L., Tu, C. F., Tsoue, H. L., Huang, M. C., Yu, Y. C., Huang, T. H. and Lin, E. C. (2012). Effects of single-nucleotide polymorphisms in insulin-like growth factor-1 and insulin-like growth factor-2 genes on growth performance of centrally tested Duroc boars using segregated early weaning entrance. Livestock Sci 144(3): 290-293.
  17. Tai, H. C., Tu, C. F., Yang, T. S., Lee, J. M., Huang, S. Y., and Wang, B. T. (2012). Function Measurement of HLA-II Transgenic Pigs for Xenotransplantation. In: Miyagawa, S. (Ed). Xenotransplantation, Intech Croatia, 55-66.
  18. Chawalit, S., Nguyen, N. T., Tseng, J. K., Lo, N. W., Tu, C. F. and Ju, J. C. (2012). Trichostatin A and ascorbic acid assist in the development of porcine handmade cloned embryos via different physiologic pathways. Reprod Sci 19(9): 976-986.
  19. Tu, C. F., Yang, T. S. and Tai, H. C. (2012). Attenuating porcine xenograft rejections by production of transgenic and cloning the genetically engineered pigs. J Chin Soc Anim Sci 41: 237-260.
  20. Wang, P. H., Lyu, Y. J., Huang, S. Y., Chien, C. C., Tu, C. F. and Lin, E. C. (2012). Preliminary study for molecular traceability of pure and hybrid pigs (fresh pork) using microsatellite markers in Taiwan. J Agri Assoc Taiwan 13(3): 296-317.
  21. Juang, J. H., Tu, C. F. and Kuo, C. H. (2011). Modest effects of Fas-ligand and heme oxygenase-1 double transgenic mouse islets on transplantation outcomes. Transplant Proc 43(9): 3198-3200.
  22. Tu, C. F., Tai, H. C., Wu, C. P., Ho, L. L., Lin, Y. J., Hwang, C. S., Yang, T. S., Lee, J. M., Tseng, Y. L., Huang, C. C., Weng, C. N. and Lee, P. H. (2010). The in vitro protection effect of hDAF and hHO-1 transgenes in porcine aortic endothelial cells against sera of Formosan macaques. Transplant Proc 42(6): 2138-2141.
  23. Yen, S. Y., Lee, S. M., Tu, C. F., Tang, S. M. and Tapsoba, J. D. (2010). A survey of the attitudes of scientists toward xenotransplantation in Taiwan. Transplant Proc 42(6): 2117-2121.
  24. Lee, K. H., Chuang, C. k. and Tu, C. F. (2009). Protein-induced pluripotent stem cells. J Chin Soc Anim Sci 38(4): 217-233.
  25. Tu, C. F., Tai, H. C., Yang, T. S., Lee, J. M., Tseng, Y. L., Chou, N. K., Yang, C. Y., Chuang, C. k., Weng, C. N. and Lee, P. H. (2009). Porcine genetic modification for xenotransplantation. Formosan J Med 13: 298-308.
  26. Yen, C. H., Yang, C. K., Chen, I. C., Lin, Y. S., Lin, C. S., Chu, S. and Tu, C. F. (2008). Expression of recombinant Hirudin in transgenic mice milk driven by the goat beta-casein promoter. Biotechnol J 3(8): 1067-1077.
  27. Lee, J. M., Tu, C. F., Tai, H. C., Chou, N. K., Weng, C. N., Lee, Y. C., and Lee, P. H. (2008). The impacts of human ABO blood groups on human xenoreactive antibody-mediated cytotoxicity and the protective effect of human decay accelerating factors (hDAF) exogene on the swine endothelium. Transplant Proc 40(2): 551-553.
  28. Tai, H. C., Tu, C. F., Lee, J. M., Ho, L. L., Tseng, Y. L., Chou, N. K., Yuang, T. S., Weng, C. N., Lee, P. H., Chang, K. J. and Tang, Y. B. (2008). Long-Term Survival of HLA-DR15+ Pig Skin in SCID Mice after Reconstitution with Human PBMC and Short-Term Immunosuppression. Transplant Proc 40(2): 570-573.
  29. Tu, C. F., Tai, H. C., Chen, C. M., Huang, T. T., Lee, J. M., Yang, T. S., Chen, C. H., Tseng, Y. L., Chou, N. K. and Lee, P. H. (2008). Human leukocyte antigen-DR matching improved skin graft survival from transgenic pigs to accommodate SCID mice reconstituted with human peripheral blood mononuclear cells. Transplant Proc 40(2): 578-580.
  30. Sun, Y. L., Chou, Y. C., Kuan, T. C., Tu, C. F. and Lin, C. S. (2008). Expression of recombinant anticoagulant hirudin in the differentiated cultures of the porcine mammary epithelial cell line SI-PMEC. Cell Biol Int 32(7): 739-747.
  31. Chen, C. H., Stone, L., Ju, J. C., Lien, W. T., Liu, M. S., Tu, C. F. and Lee, K. H. (2008). Transgenic cloned mice expressing enhanced green fluorescent protein generated by activation stimuli combined with 6-dimethylaminopurine. Reprod Domest Anim 43(5): 547-555.
  32. Lee, K. H., Chuang, C. K., Wang, H. W., Stone, L., Chen, C. H. and Tu, C. F. (2007). An alternative simple method for mass production of chimeric embryos by coculturing denuded embryos and embryonic stem cells in Eppendorf vials. Theriogenology 67(2): 228-237.
  33. Tai, H. C., Lee, J. M., Chou, N. K., Tu, C. F. and Lee, P. H. (2007). Xenotransplantation: an example of aphla-Gal gene kockout pigs. Formosan J Med 11(1): 70-78.
  34. Huang, S. Y., Chen, Y. H., Teng, S. H., Chen, I. C., Ho, L. L. and Tu, C. F. (2006). Protein expression of lymphocytes in HLA-DR transgenic pigs by a proteomic approach. Proteomics 6(21): 5815-5825.
  35. Lee, J. M., Tu, C. F., Tai, H. C., Chou, N. K., Yang, T. S., Weng, C. N., Lee, Y. C., Lee, C. J. and Lee, P. H. (2006). The hDAF exogene protects swine endothelial and peripheral blood mononuclear cells from xenoreactive antibody mediated cytotoxicity in hDAF transgenic pigs. Transplant Proc 38(7): 2270-2272.
  36. Huang, S. Y., Tu, C. F., Liu, S. H. and Kuo, Y. H. (2005). Motility and fertility of alginate encapsulated boar spermatozoa. Anim Reprod Sci 87(1-2): 111-120.
  37. Chen, M. Y., Tu, C. F., Wuang, S. Y., Lin, J. H., Tzang, B. S., Tsou, H. L., Hseu, T. H. and Lee, W. C. (2005). Augmentation thermotolerance in primary skin fibroblasts from a transgenic pig overexpressing the porcine HSP70.2. Asian-Australia J Anim Sci 18(1): 107-112.
  38. Tu, C. F., Kuo, C. H. and Juang, J. H. (2005). Effects of heme oxygenase-1 transgenic islets on transplantation. Transplant Proc 37(8): 3463-3467.
  39. Tu, C. F., Huang, K. F., Chen, I. C., Lin, C. J., Wu, S. C. and Yang, P. C. (2004). Application of real time PCR to rapidly screen Hirudin transgenic piglets. J Chin Soc Anim Sci 33(4): 1-12.
  40. Wang, B. T., Tu, C. F., Hsieh, L. J., Tai, H. C., Chiu, Y. L., Lee, J. M., Kuo, S. J., Tsuji, K. and Lee, C. J. (2004). Rapid detection of human HLA transgenes in pigs by fluorescence in situ hybridization (FISH) for adjuvant study of human xenotransplantation. Xenotransplantation 11(5): 471-475.
  41. Tu, C. F., Lee, J. M., Sato, T., Dai, H. C., Lee, F. R., Yang, C. K., Tsuji, K. and Lee, C. J. (2003). Expression of HLA-DQ genes in transgenic pigs. Transplant Proc 35(1): 513-515.
  42. Lee, J. M., Tu, C. F., Huang, S. C., Tsuji, K., Chen, R. J., Hu, C. Y., Hsieh, R. P., Tai, H. C., Weng, C. N., Lee, Y. C. and Lee, C. J. (2003). Attenuation of human-to-pig xenogenic cellular proliferation and Th1 response by expressing the human MHC II DQ exogenes on porcine cells. Transplant Proc 35(1): 527-528.
  43. Lee, J. W., Wu, S. C., Tian, X. C., Barber, M., Hoagland, T., Riesen, J., Lee, K. H., Tu, C. F., Cheng, W. T. and Yang, X. (2003). Production of cloned pigs by whole-cell intracytoplasmic microinjection. Biol Reprod 69(3): 995-1001.
  44. Tu, C. F., Liu, M. S. and Kuo, Y. H. (2003). A simple vitrification procedure for cryopreservation of transgenic mouse embgryos. J Chin Soc Anim Sci 32 (1): 33-42.
  45. Lee, K. H., Tu, C. F., Wu, S. C. and Hsu, W. C. (2003). Routine generation of green fluorescent chimeric mice with a high percentage of germline transmission by a novel embryonic stem cell line. J Chin Soc Anim Sci 32 (2): 143-154.
  46. Lee, J. M., Tu, C. F., Yang, P. W., Lee, K. H., Tsuji, K., Tsai, M. K., Chen, R. J., Hu, C. Y., Hsieh, R. P., Tai, H. C., Chiang, B. L., Weng, C. N., Lee, Y. C. and Lee, C. J. (2002). Reduction of human-to-pig cellular response by alteration of porcine MHC with human HLA DPW0401 exogenes. Transplantation 73(2): 193-197.
  47. Juang, J. H., Tu, C. F., Kuo, C. H. and Hsu, P. N. (2002). Failure of Fas-ligand transgenic islets to resist allogeneic rejection. Transplant Proc 34(5): 1456-1457.
  48. Hsu, P. N., Lin, H. H., Tu, C. F., Chen, N. J., Wu, K. M., Tsai, H. F. and Hsieh, S. L. (2001). Expression of human Fas ligand on mouse beta islet cells does not induce insulitis but is insufficient to confer immune privilege for islet grafts. J Biomed Sci 8(3): 262-269.
  49. Tu, C. F. and Yang, L. L. (2001). Generation of transgenic mice carrying HLA-DR (A1+B1) and CMV-hDAF three transgenes. J Chin Soc Anim Sci 30(2): 129-140.
  50. Tu, C. F., Wu, S. C., Yang, P. C. and Cheng, W. T. K. (2001). Technology and application of transgenesis in livestock. J Chin Soc Anim Sci 30(1): 37-43.
  51. Huang, H. W., Liu, T. Z., Lee, K. H., Tu, C. F., Lee, W. C., Shimogiri, T., Mannen, H. and Li, S. S. (2000). cDNA cloning of pig testicular lactate dehydrogenase-C, thermal stablility of the expressed enzyme, and polymorphism among strains. Gene 242(1-2): 151-154.
  52. Tu, C. F., Hsieh, S. L., Lee, J. M., Yang, L. L., Sato, T., Lee, K. H., Weng, C. N., Mao, S. J., Tsuji, K. and Lee, C. J. (2000). Successful generation of transgenic pigs for human decay-accelerating factor and human leucocyte antigen DQ. Transplant Proc 32(5): 913-915.
  53. King, Y. T., Lee, W. C., Gao, M. S., Wang, J. L., Tu, C. F., Wu, S. C. and Kuo, Y. H. (2000). Synthesis of 60- and 72 kDa heat shock proteins in early porcine embryogenesis. Anim Reprod Sci 63(3-4): 221-229.
  54. Lee, J. M., Tu, C. F., Yang, P. W., Lee, Y. C. and Lee, C. J. (2000). The effective antigen presentation of human MHC on the lymphocytes of HLA DPW0401 transgenic pigs: examination with xenogenic mixed lymphocyte culture and primed lymphocyte tests. Transplant Proc 32(7): 2503-2504.
  55. Tu, C. F., Hwang, L. H. and Cheng, W. T. K. (1999). Generation of transgenic mice by subzonal infection methods after microinjection of the retroviral particles into perivitelline space of mouse early embryos. J Chin Soc Anim Sci 28(3): 379-392.
  56. Tu, C. F., Tsuji, K., Lee, K. H., Chu, R., Sun, T. J., Lee, Y. C., Weng, C. N. and Lee, C. J. (1999). Generation of HLA-DP transgenic pigs for the study of xenotransplantation. Int Surg 84(2): 176-182.
  57. Tu, C. F., Sato, T., Hagihara, M., Lee, K. H., Lee, Y. C., Weng, C. N., Chu, R. M., Tsuji, K. and Lee, C. J. (1998). Expression of HLA-DP antigen on peripheral blood mononuclear cells of HLA-DP transgenic pigs. Transplant Proc 30(7): 3502-3503.
  58. Tu, C. F., Ma, R. C. S. and Cheng, W. T. K. (1998). Effect of operation time on pronuclear embryos recover rate and relative centrifugal field on pronucleaus exposured ratio and embryo survival rate. J Chin Soc Anim Sci 27(1): 49-58.
  59. Tu, C. F., Choo, K. B. and Cheng, W. T. K. (1995). Production and analysis of transgenic pig with human factor IX gene. J Chin Soc Anim Sci 24(3): 273-287.
1 Protocol published
Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors
Authors:  Chin-kai Chuang, Ching-Fu Tu and Chien-Hong Chen, date: 06/05/2017, view: 2958, Q&A: 0
A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy. Since the de novo zygotic transcription ...