Xiaoou Jiang Microbiology & Immunology, National University of Singapore, Singapore
2 protocols

Volker Patzel
  • Microbiology & Immunology, National University of Singapore, Singapore
  • 2 Author merit


Dr. rer. nat. (Ph.D), German Cancer Research Center, Germany, 1998

Current position

Assistant Professor & PI, Microbiology & Immunology, National University of Singapore, Singapore

Assistant Director of Research, Medicine, University of Cambridge, United Kingdom


  1. Pan, H. M., Yu, H., Guigas, G., Fery, A., Weiss, M., Patzel, V. and Trau, D. (2017). Engineering and Design of Polymeric Shells: Inwards Interweaving Polymers as Multilayer Nanofilm, Immobilization Matrix, or Chromatography Resins. ACS Appl Mater Interfaces 9(6): 5447-5456.
  2. Ingemarsdotter, C. K., Poddar, S., Mercier, S., Patzel, V. and Lever, A. M. L. (2017). Expression of Herpes Simplex Virus Thymidine Kinase/Ganciclovir by RNA Trans-Splicing Induces Selective Killing of HIV-Producing Cells. Mol Ther Nucleic Acids 7: 140-154.
  3. Jiang, X., Yu, H., Teo, C. R., Tan, G. S., Goh, S. C., Patel, P., Chua, Y. K., Hameed, N. B., Bertoletti, A. and Patzel, V. (2016). Advanced Design of Dumbbell-shaped Genetic Minimal Vectors Improves Non-coding and Coding RNA Expression. Mol Ther 24(9): 1581-1591.
  4. Yu, H., Jiang, X., Tan, K. T., Hang, L. and Patzel, V. (2015). Efficient production of superior dumbbell-shaped DNA minimal vectors for small hairpin RNA expression. Nucleic Acids Res 43(18): e120.
  5. Patzel, V. (2015). Implementation of a Technology-supported Three-stage Classroom Feedback System for Promotion of Self-regulation and Assessment of Student and Teacher Performance. American Journal of Educational Research 3(4): 446-449.
  6. Tan, K. S., Choi, H., Jiang, X., Yin, L., Seet, J. E., Patzel, V., Engelward, B. P. and Chow, V. T. (2014). Micro-RNAs in regenerating lungs: an integrative systems biology analysis of murine influenza pneumonia. BMC Genomics 15: 587.
  7. Poddar, S., Eul, J. and Patzel, V. (2014). Homologous SV40 RNA trans-splicing: Special case or prime example of viral RNA trans-splicing? Comput Struct Biotechnol J 10(16): 51-57.
  8. Patzel, V. (2014). The role of guide RNA structure in RNA interference. Journal of Microbiology & Experimentation 1(1): 1-2. 
  9. Jung, U., Jiang, X., Kaufmann, S. H. and Patzel, V. (2013). A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA. RNA 19(12): 1864-1873.
  10. Patzel, V. (2013). Viral RNA trans-splicing: Chance event or product of evolution? SOJ Microbiology and Infectious Diseases 1(1): 1-2.
  11. Eul, J. and Patzel, V. (2013). Homologous SV40 RNA trans-splicing: a new mechanism for diversification of viral sequences and phenotypes. RNA Biol 10(11): 1689-1699.
  12. Patzel, V. (2009). Molecular design for genetic therapy: Computer-aided nucleic acid design for gene and cell therapy to treat type 2 Gaucher’s disease. Transfer 5-6.
  13. Mantei, A., Rutz, S., Janke, M., Kirchhoff, D., Jung, U., Patzel, V., Vogel, U., Rudel, T., Andreou, I., Weber, M. and Scheffold, A. (2008). siRNA stabilization prolongs gene knockdown in primary T lymphocytes. Eur J Immunol 38(9): 2616-2625.
  14. Patzel, V. (2008). siRNA/RNAi: ein neuer Therapieansatz? Fortschritte der praktischen Dermatologie und Venerologie 21: 7-12.
  15. Patzel, V. (2007). In silico selection of active siRNA. Drug Discov Today 12(3-4): 139-148.
  16. Köberle, C., Jung, U., Dietrich, I., Kaufmann, S. H. E. and Patzel, V. (2007). siRNA: structure-based design and prokaryotic gene silencing. Journal of RNAi and gene silencing.
  17. Good, L. and Stach, J. E. (2011). Synthetic RNA silencing in bacteria - antimicrobial discovery and resistance breaking. Front Microbiol 2: 185.
  18. Patzel, V., Dietrich, I. and Kaufmann, S. H. (2006). RNA Silencing in the struggle against disease. Ann N Y Acad Sci 1082: 44-46.
  19. Koberle, C., Kaufmann, S. H. and Patzel, V. (2006). Selecting effective siRNAs based on guide RNA structure. Nat Protoc 1(4): 1832-1839.
  20. Patzel, V., Rutz, S., Dietrich, I., Koberle, C., Scheffold, A. and Kaufmann, S. H. (2005). Design of siRNAs producing unstructured guide-RNAs results in improved RNA interference efficiency. Nat Biotechnol 23(11): 1440-1444.
  21. Patzel, V. and Kaufmann, S. H. (2003). Towards simple artificial infectious systems. Trends Mol Med 9(11): 479-482.
  22. Lehmann, M. J., Patzel, V. and Sczakiel, G. (2000). Theoretical design of antisense genes with statistically increased efficacy. Nucleic Acids Res 28(13): 2597-2604.
  23. Patzel, V. and Sczakiel, G. (2000). In vitro selection supports the view of a kinetic control of antisense RNA-mediated inhibition of gene expression in mammalian cells. Nucleic Acids Res 28(13): 2462-2466.
  24. Scherr, M., Rossi, J. J., Sczakiel, G. and Patzel, V. (2000). RNA accessibility prediction: a theoretical approach is consistent with experimental studies in cell extracts. Nucleic Acids Res 28(13): 2455-2461.
  25. Patzel, V. and Sczakiel, G. (1999). Length dependence of RNA-RNA annealing. J Mol Biol 294(5): 1127-1134.
  26. Patzel, V., Steidl, U., Kronenwett, R., Haas, R. and Sczakiel, G. (1999). A theoretical approach to select effective antisense oligodeoxyribonucleotides at high statistical probability. Nucleic Acids Res 27(22): 4328-4334.
  27. Langle-Rouault, F., Patzel, V., Benavente, A., Taillez, M., Silvestre, N., Bompard, A., Sczakiel, G., Jacobs, E. and Rittner, K. (1998). Up to 100-fold increase of apparent gene expression in the presence of Epstein-Barr virus oriP sequences and EBNA1: implications of the nuclear import of plasmids. J Virol 72(7): 6181-6185.
  28. Patzel, V. and Sczakiel, G. (1998). Theoretical design of antisense RNA structures substantially improves annealing kinetics and efficacy in human cells. Nat Biotechnol 16(1): 64-68.
  29. Patzel, V., Kronenwett, R., Rittner, K. and Sczakiel, G. (1997). Fast RNA-RNA annealing in vitro: single cycle selection versus multiple cycle selection. Nucleotides & Nucleosides 16(5&6): 711-715.
  30. Patzel, V., zu Putlitz, J., Wieland, S., Blum, H. E. and Sczakiel, G. (1997). Theoretical and experimental selection parameters for HBV-directed antisense RNA are related to increased RNA-RNA annealing. Biol Chem 378(6): 539-543.
  31. Patzel, V. and Sczakiel, G. (1997). The hepatitis B virus posttranscriptional regulatory element contains a highly stable RNA secondary structure. Biochem Biophys Res Commun 231(3): 864-867.
  32. List, H. J., Patzel, V., Zeidler, U., Schopen, A., Ruhl, G., Stollwerk, J. and Klock, G. (1994). Methylation sensitivity of the enhancer from the human papillomavirus type 16. J Biol Chem 269(16): 11902-11911.
2 Protocols published
Advanced Design of Minimalistic Dumbbell-shaped Gene Expression Vectors
Authors:  Xiaoou Jiang and Volker Patzel, date: 08/05/2017, view: 1347, Q&A: 0
Minimal DNA vectors exclusively comprising therapeutically relevant sequences hold great promise for the development of novel therapeutic regimen. Dumbbell-shaped vectors represent non-viral non-integrating DNA minimal vectors which have entered an ...
Formation of Minimised Hairpin Template-transcribing Dumbbell Vectors for Small RNA Expression
Authors:  Xiaoou Jiang and Volker Patzel, date: 06/05/2017, view: 1772, Q&A: 0
A major barrier for using non-viral vectors for gene therapy is the short duration of transgene expression in postmitotic tissues. Previous studies showed transgene expression from conventional plasmid fell to sub-therapeutic level shortly after ...