Satyakam Patnaik —BIO-PROTOCOL

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Debabrata Ghosh Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, India
1 protocol

Mahaveer Purohit Water Analysis Laboratory, Nanotherapeutics and Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, India
1 protocol

Ruchi Gera Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, India
1 protocol

Vikas Singh Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, India
1 protocol

Satyakam Patnaik

Water Analysis Laboratory, Nanotherapeutics and Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, India

Research focus

Cancer biology

1 Author merit
Reviewer merit
Editor merit

Education

Ph.D, Delhi University, India, 2007

Current position

Scientist, Nanotherapeutics and Nanomaterial Toxicology, CSIR-Indian Institution of Toxicology Research, India

Publications

https://scholar.google.com/citations?hl=en&user=Lo27JIUAAAAJ

1 Protocol published

Fluorometric Estimation of Glutathione in Cultured Microglial Cell Lysate

Authors: Vikas Singh, Ruchi Gera, Mahaveer Prasad Purohit, Satyakam Patnaik and Debabrata Ghosh, date: 06/05/2017, view: 8618, Q&A: 6

Glutathione is one of the major antioxidant defense components present in cells. It is predominantly present as reduced glutathione (GSH) and converted into oxidized glutathione (GSSG) while reducing the free radicals like hydroxyl ions (OH^- ...

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LIAO liping

Shanghai Insitute of Materia Medica

Hello！ I still have another question,GSSG is hydrolysis to two molar GSH ,wich means at the standard curve the same concentration of GSSG and GSH, GSSG will have almost two times fluorecence signal than GSH ,but as standard curve of yours and mine shows that it is not? How to explain those phenomenon. Thanyou

10/22/2019 2:06:00 AM Reply

Vikas Singh

Immunotoxicology Laboratory, Food Drug and Chemical Toxicology Group and Nanotherapeutics & Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31-Vishvigyan Bhawan, India

Hi, I think it is really difficult to get the fluorescence in such a way that GSSG standard show double the fluorescence of GSH standard. This may be due to the rapid oxidation of GSH once it comes in solution form due to oxidizing environment which might also play a role in converting GSH into GSSG.

2019-10-23 11:41:35