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A peer-reviewed protocol journal. No publication fee; no access fee.
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Zhaoxia Qu
University of Pittsburgh Cancer Institute, University of Pittsburgh, USA
2 protocols
Gutian Xiao
University of Pittsburgh Cancer Institute, University of Pittsburgh, USA
2 protocols
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Reviewer
Fan Sun
University of Pittsburgh
Research focus
Cancer biology
Contributions
2
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2 Protocols published
Isolation of Murine Alveolar Type II Epithelial Cells
Authors:
Fan Sun
,
Gutian Xiao
and
Zhaoxia Qu
,
date:
05/20/2017,
view:
5410,
Q&A:
0
We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar ...
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Murine Bronchoalveolar Lavage
Authors:
Fan Sun
,
Gutian Xiao
and
Zhaoxia Qu
,
date:
05/20/2017,
view:
11535,
Q&A:
1
A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, ...
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Caroline Nijskens
Centre d'économie rural
We tried this method only one time...but It was probably due to less experience;..we must try it back later.
12/2/2018 11:36:14 PM
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