Laura Molina-García Centro de Investigaciones Biológicas-CSIC
2 protocols
Christian Roth University of York
6 protocols

David Cisneros Umeå University
33 protocols

Maria Sinetova Laboratory of Intracellular Regulation, Institute of Plant Physiology, Russian Academy of Sciences, Russia
45 protocols

Yanjie Li Yale University
30 protocols

Damián Lobato-Márquez
  • Research associate, Imperial College London
Research focus
  • Microbiology
  • 1 Author merit


PhD, Autonoma University, 2015

1 Protocol published
Evaluation of Plasmid Stability by Negative Selection in Gram-negative Bacteria
Authors:  Damián Lobato Márquez and Laura Molina García, date: 05/05/2017, view: 10172, Q&A: 0
Plasmid stability can be measured using antibiotic-resistance plasmid derivatives by positive selection. However, highly stable plasmids are below the sensitivity range of these assays. To solve this problem we describe a novel, highly sensitive ...
3 Protocols reviewed
In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones
Authors:  Rachel Fellows and Patrick Varga-Weisz, date: 07/20/2018, view: 4938, Q&A: 0
Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an in vitro approach to assaying class I HDACs with ...
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In vitro Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease
Authors:  Sakshi Chauhan, Gajendra Kumar Azad and Raghuvir Singh Tomar, date: 01/05/2017, view: 7740, Q&A: 0
Histone proteins are subjected to a wide array of reversible and irreversible post-translational modifications (PTMs) (Bannister and Kouzarides, 2011; Azad and Tomar, 2014). The PTMs on histones are known to regulate chromatin structure and ...
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