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A peer-reviewed protocol journal. No publication fee; no access fee.
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Laura Molina-García
Centro de Investigaciones Biológicas-CSIC
2 protocols
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Christian Roth
University of York
6 protocols
David Cisneros
Umeå University
33 protocols
Maria Sinetova
Laboratory of Intracellular Regulation, Institute of Plant Physiology, Russian Academy of Sciences, Russia
45 protocols
Yanjie Li
Yale University
30 protocols
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Reviewer
Damián Lobato-Márquez
Research associate, Imperial College London
Research focus
Microbiology
Contributions
1
Author merit
Reviewer merit
Editor merit
Education
PhD, Autonoma University, 2015
1 Protocol published
Evaluation of Plasmid Stability by Negative Selection in Gram-negative Bacteria
Authors:
Damián Lobato Márquez
and
Laura Molina García
,
date:
05/05/2017,
view:
8779,
Q&A:
0
Plasmid stability can be measured using antibiotic-resistance plasmid derivatives by positive selection. However, highly stable plasmids are below the sensitivity range of these assays. To solve this problem we describe a novel, highly sensitive ...
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3 Protocols reviewed
In vitro
Enzymatic Assays of Histone Decrotonylation on Recombinant Histones
Authors:
Rachel Fellows
and
Patrick Varga-Weisz
,
date:
07/20/2018,
view:
3804,
Q&A:
0
Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an
in vitro
approach to assaying class I HDACs with ...
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In vitro
Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease
Authors:
Sakshi Chauhan
,
Gajendra Kumar Azad
and
Raghuvir Singh Tomar
,
date:
01/05/2017,
view:
6735,
Q&A:
0
Histone proteins are subjected to a wide array of reversible and irreversible post-translational modifications (PTMs) (Bannister and Kouzarides, 2011; Azad and Tomar, 2014). The PTMs on histones are known to regulate chromatin structure and ...
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