Vincent Rotello University of Massachusetts, USA
2 protocols

Rubul Mout
  • University of Washington , USA
  • 2 Author merit

Education

PhD, University of Massachusetts Amherst, USA, 2017

Current position

WRF Innovation Fellow, School of Medicine, University of Washington, USA

Publications

  1. https://scholar.google.com/citations?user=NpMfqDUAAAAJ&hl=en
2 Protocols published
A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 12/20/2017, view: 2038, Q&A: 0
In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using ...
Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 3952, Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.