Search
Coauthors
Vincent Rotello University of Massachusetts, USA
2 protocols

Rubul Mout
  • University of Washington , USA
  • 2 Author merit

Education

PhD, University of Massachusetts Amherst, USA, 2017

Current position

WRF Innovation Fellow, School of Medicine, University of Washington, USA

Publications

  1. https://scholar.google.com/citations?user=NpMfqDUAAAAJ&hl=en
2 Protocols published
A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 12/20/2017, view: 1797, Q&A: 0
In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using ...
Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 3451, Q&A: 0
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...