Alessandro Didonna University of California, San Francisco
36 protocols

Jingli Cao Duke University Medical Center
11 protocols

José Dias Karolinska Institutet
2 protocols

Kae-Jiun Chang University of California, San Francisco
12 protocols

Editor
Salome Botelho
  • Stanford University
Research focus
  • Biochemistry
5 Protocols reviewed
Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
Authors:  Katarina Trajkovic, Hyunkyung Jeong and Dimitri Krainc, date: 01/05/2018, view: 4348, Q&A: 0
Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of ...
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The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level
Authors:  Amandine Bastide, Jonathan W. Yewdell and Alexandre David, date: 01/05/2018, view: 7760, Q&A: 0
While isotopic labeling of amino acids remains the reference method in the field for quantifying translation rate, it does not provide any information on spatial localization of translation sites. The rationale behind developing the ...
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