Anastasia D Gazi
  • Institut Pasteur
Research focus
  • Microbiology
1 Protocol reviewed
Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila for Negative Stain Electron Microscopy Studies
Authors:  Tomoko Kubori and Hiroki Nagai, date: 04/20/2017, view: 1452, Q&A: 0
Legionella possesses a pivotal secretion machinery to deliver virulence factors to eukaryotic host cells. In this protocol, we describe the procedure for isolation of the native core complex of the Dot/Icm type IV secretion system from L. ...
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