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A peer-reviewed protocol journal. No publication fee; no access fee.
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Reviewer
Anastasia D Gazi
Institut Pasteur
Research focus
Microbiology
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1 Protocol reviewed
Isolation of the Dot/Icm Type IV Secretion System Core Complex from
Legionella pneumophila
for Negative Stain Electron Microscopy Studies
Authors:
Tomoko Kubori
and
Hiroki Nagai
,
date:
04/20/2017,
view:
3405,
Q&A:
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Legionella
possesses a pivotal secretion machinery to deliver virulence factors to eukaryotic host cells. In this protocol, we describe the procedure for isolation of the native core complex of the Dot/Icm type IV secretion system from
L.
...
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