Anastasia D Gazi —BIO-PROTOCOL

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Anastasia D Gazi

Institut Pasteur

Research focus

Microbiology

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1 Protocol reviewed

Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila for Negative Stain Electron Microscopy Studies

Authors: Tomoko Kubori and Hiroki Nagai, date: 04/20/2017, view: 2710, Q&A: 0

Legionella possesses a pivotal secretion machinery to deliver virulence factors to eukaryotic host cells. In this protocol, we describe the procedure for isolation of the native core complex of the Dot/Icm type IV secretion system from L. ...

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