Alba Blesa Universidad Francisco de Vitoria
29 protocols

Arsalan Daudi University of California
157 protocols

Cindy Cooke Carnegie Institution for Science
11 protocols

Cristina Colomer-Winter Boehringer Ingelheim
5 protocols

Editor
Wenrong He
  • Post-Doc, Salk Institute for Biological Studies
Research focus
  • Plant science
  • miRNA biogenesis, activity and turnover
    Translational regulation
    Phytohormone signaling and response
    System genetics and chemical genetics

Education

Ph.D., Peking Univeristy

Lab information

Wolfgang Busch lab

Publications

1. Cai Q, Liang C, Gao L, Liu L, He W, Mo B, Chen X. The disease resistance protein SNC1 represses the biogenesis of microRNAs and phased siRNAs. Nature Communications. 2018. https://doi.org/10.1038/s41467-018-07516-z
2. Sun X #, Li Y #, He W, Ji C, Xia P, Wang Y, Du S, Li H, Raikhel N, Xiao J, Guo H. Identification of pyrazinamide and its derivatives as potent and specific inhibitors of ACC oxidase in ethylene biosynthesis. Nature Communications. 2017. doi: 10.1038/ncomms15758. (# co-first authors)
3. Zhang X, Chen Y, Lin X, Hong X, Zhu Y, Li W, He W, An F, Guo H.Adenine phosphoribosyl transferase 1 is a key enzyme catalyzing cytokinin conversion from nucleobases to nucleotides in Arabidopsis. Molecular Plant. 2013, 6(5):1661-72
4. Wen X, Zhang C, Ji Y, Zhao Q, He W, An F, Jiang L, Guo H. Activation of Ethylene Signaling Is Mediated by Nuclear Translocation of the Cleaved EIN2 Carboxyl Terminus. Cell Research. 2012, 22(11): 1613-6
5. An F, Zhang X, Zhu Z, Ji Y, He W, Jiang Z, Li M, Guo H. Coordinated regulation of apical hook development by gibberellins and ethylene in etiolated Arabidopsis seedlings. Cell Research. 2012, 22(5): 915-27
6. He W, Brumos J, Li H, Ji Y, Ke M, Gong X, Zeng Q, Li W, Zhang X, An F, Wen X, Li P, Chu J, Sun X, Yan C, Yan N, Xie DY, Raikhel N, Yang Z, Stepanova AN, Alonso JM, Guo H. A Small-Molecule Screen Identifies L-Kynurenine as a Competitive Inhibitor of TAA1/TAR Activity in Ethylene-Directed Auxin Biosynthesis and Root Growth in Arabidopsis. The Plant Cell. 2011, 23(11):3944-60
7. Stepanova AN, Yun J, Robles LM, Novak O, He W, Guo H, Ljung K, Alonso JM. The Arabidopsis YUCCA1 Flavin Monooxygenase Functions in the Indole-3-Pyruvic Acid Branch of Auxin Biosynthesis. The Plant Cell. 2011, 23(11):3961-73
8. Jiang Z, Liu X, Peng Z, Wan Y, Ji Y, He W, Wan W, Luo J, Guo H. AHD2.0: an update version of Arabidopsis Hormone Database for plant systematic studies. Nucleic Acids Research. 2011, 39(Database issue):D1123-9
9. Zhao Q, He W, Zhang X, Guo H. Genetic and biochemical study of the ethylene signaling in Arabidopsis. Chinese Bulletin of Life Sciences. 2010, 22(11):1167-72
10. An F, Zhao Q, Ji Y, Li W, Jiang Z, Yu X, Zhang C, Han Y, He W, Liu Y, Zhang S, Ecker JR, Guo H. Ethylene-induced stabilization of ETHYLENE INSENSITIVE3 and EIN3-LIKE1 is mediated by proteasomal degradation of EIN3 binding F-box 1 and 2 that requires EIN2 in Arabidopsis. The Plant Cell. 2010, 22(7):2384-401
9 Protocols reviewed
Determination of Hydrodynamic Radius of Proteins by Size Exclusion Chromatography
Authors:  Valentina La Verde, Paola Dominici and Alessandra Astegno, date: 04/20/2017, view: 27186, Q&A: 0
Size exclusion chromatography (SEC) or gel filtration is a hydrodynamic technique that separates molecules in solution as a function of their size and shape. In the case of proteins, the hydrodynamic value that can be experimentally derived is the ...
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Putrescine Biosynthesis Inhibition in Tomato by DFMA and DFMO Treatment
This protocol can be used to inhibit the biosynthesis of polyamines, specifically putrescine, in tomato plants grown with NH4+ as a solely N source. In general, polyamines are positively charged small metabolites implicated in ...
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4 Protocols edited
Measurements of Root Colonized Bacteria Species
Authors:  Vílchez Juan Ignacio, Yu Yang, Dian Yi and Huiming Zhang, date: 04/05/2021, view: 2144, Q&A: 0

Root-associated bacteria are able to influence plant fitness and vigor. A key step in understanding the belowground plant-bacteria interactions is to quantify root colonization by the bacteria of interest. Probably, genetic engineering with

...
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In vitro Reconstitution Assays of Arabidopsis 20S Proteasome
Authors:  Yanjun Li, Di Sun, Xingxing Yan, Zhiye Wang and Xiuren Zhang, date: 04/05/2021, view: 2941, Q&A: 0

The majority of cellular proteins are degraded by the 26S proteasome in eukaryotes. However, intrinsically disordered proteins (IDPs), which contain large portions of unstructured regions and are inherently unstable, are degraded via the

...
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