Arsalan Daudi University of California
157 protocols

Kenji Sugiyama University of Tsukuba
1 protocol

Stéphane Romero
  • Ecole Polytechnique
Research focus
  • Cell biology
1 Protocol reviewed
Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor
Authors:  Rajan Lamichhane, Jeffrey J. Liu, Raymond F. Pauszek III and David P. Millar, date: 06/20/2017, view: 4895, Q&A: 0
Activation of G protein-coupled receptors (GPCRs) by agonist ligands is mediated by a transition from an inactive to active receptor conformation. We describe a novel single-molecule assay that monitors activation-linked conformational transitions ...
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