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Coreviewers
Anca Savulescu The Council for Scientific and Industrial Research
4 protocols

Chao Jiang Stanford University
2 protocols

Samik Bhattacharya Osnabrueck University
49 protocols

Scott McAdam Purdue University
23 protocols

Reviewer
Yi Zhang
  • Post-Doc, University of Nebraska-Lincoln
Research focus
  • Molecular biology
  • Plant immunity, Ubiquitination
  • 1 Author merit

Education

Ph.D., University of Wuerzburg, 2014

Lab information

Department of Plant Pathology
University of Nebraska-Lincoln

1 Protocol published
5’ Rapid Amplification of cDNA Ends (5’ RACE) of Agrobacterial T-DNA Genes within Transformed Plant Sample
Author:  Yi Zhang, date: 09/20/2015, view: 3440, Q&A: 0
The T-DNA (transferred-DNA) region of virulent Agrobacterium tumefaciens (A. tumefaciens) strain is transferred and integrated into the plant genome, and thereby the T-DNA genes are expressed in transformed plant cells. This ...
4 Protocols reviewed
Isolation of Guard-cell Enriched Tissue for RNA Extraction
Authors:  Pirko Jalakas, Dmitry Yarmolinsky , Hannes Kollist and Mikael Brosche, date: 08/05/2017, view: 1392, Q&A: 0
This is a protocol for isolation of guard cell enriched samples from Arabidopsis thaliana plants for RNA extraction. Leaves are blended in ice-water and filtered through nylon mesh to obtain guard cell enriched fragments. With guard cell ...
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Single Genome Sequencing of Expressed and Proviral HIV-1 Envelope Glycoprotein 120 (gp120) and nef Genes
The current study provides detailed protocols utilized to amplify the complete HIV-1 gp120 and nef genes from single copies of expressed or integrated HIV present in fresh-frozen autopsy tissues of patients who died while on ...
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