Anca Savulescu University of Cape Town
14 protocols

Chao Jiang Stanford University
4 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
42 protocols

Samik Bhattacharya Osnabrueck University
55 protocols

Reviewer
Yi Zhang
  • Post-Doc, University of Nebraska-Lincoln
Research focus
  • Molecular biology
  • Plant immunity, Ubiquitination
  • 1 Author merit

Education

Ph.D., University of Wuerzburg, 2014

Lab information

Department of Plant Pathology
University of Nebraska-Lincoln

1 Protocol published
5’ Rapid Amplification of cDNA Ends (5’ RACE) of Agrobacterial T-DNA Genes within Transformed Plant Sample
Author:  Yi Zhang, date: 09/20/2015, view: 5731, Q&A: 0
The T-DNA (transferred-DNA) region of virulent Agrobacterium tumefaciens (A. tumefaciens) strain is transferred and integrated into the plant genome, and thereby the T-DNA genes are expressed in transformed plant cells. This ...
5 Protocols reviewed
Visualization of RNA 3’ ends in Escherichia coli Using 3’ RACE Combined with Primer Extension
Authors:  Xun Wang, Heung Jin Jeon, Monford Paul Abishek N, Jin He and Heon M. Lim, date: 03/05/2018, view: 1507, Q&A: 0
In this assay, 3’ RACE (Rapid Amplification of cDNA 3’ Ends) followed by PE (primer extension), abbreviated as 3’ RACE-PE is used to identify the mRNA 3’ ends. The following protocol describes the amplification of the mRNA 3’ ends at the galactose ...
More >
Isolation of Guard-cell Enriched Tissue for RNA Extraction
Authors:  Pirko Jalakas, Dmitry Yarmolinsky , Hannes Kollist and Mikael Brosche, date: 08/05/2017, view: 6754, Q&A: 0
This is a protocol for isolation of guard cell enriched samples from Arabidopsis thaliana plants for RNA extraction. Leaves are blended in ice-water and filtered through nylon mesh to obtain guard cell enriched fragments. With guard cell ...
More >
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.