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A peer-reviewed protocol journal. No publication fee; no access fee.
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Coreviewers
Arsalan Daudi
University of California
157 protocols
Marc-Antoine Sani
University of Melbourne
11 protocols
Saswata Sankar Sarkar
Stanford University
2 protocols
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Reviewer
Ching Yao Yang
New York University
Research focus
Biochemistry
Contributions
Reviewer merit
Editor merit
3 Protocols reviewed
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
Authors:
Akira Karasawa
and
Toshimitsu Kawate
,
date:
09/05/2017,
view:
2850,
Q&A:
0
The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett
et al
., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...
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Preparation of Everted Membrane Vesicles from
Escherichia coli
Cells
Author:
Marina Verkhovskaya
,
date:
05/05/2017,
view:
2277,
Q&A:
0
The protocol for obtaining electrically sealed membrane vesicles from
E. coli
cells is presented. Proton pumps such as Complex I, quinol oxidase, and ATPase are active in the obtained vesicles. Quality of the preparation was tested by ...
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