Emilie Viennois —BIO-PROTOCOL

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Didier Merlin Institute for Biomedical Science, Georgia state university, United States
4 protocols

Chunhua Yang Institute for Biomedical Science, Georgia state university, United States
3 protocols

Junsik Sung Institute for Biomedical Science, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA
4 protocols

Mingzhen Zhang Georgia State University, USA
2 protocols

Ana Almeida University College Cork
7 protocols

Andrea Puhar MIMS and Department of Molecular Biology, Umeå University
51 protocols

Anna Zorina Russian Academy of Sciences
5 protocols

Kathrin Sutter University of Duisburg-Essen
15 protocols

Reviewer

Emilie Viennois

Assistant Professor, INSERM

Research focus

Molecular biology
Inflammatory Bowel diseases
Cancer
Microbiota

3 Author merit
Reviewer merit
Editor merit

Education

PhD, Universite Blaise Pascal, France, 2011

Publications

https://scholar.google.com/citations?user=byweUwIAAAAJ&hl=en

3 Protocols published

Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of Zingiber officinale

Authors: Junsik Sung, Chunhua Yang, Emilie Viennois, Mingzhen Zhang and Didier Merlin, date: 10/05/2019, view: 3132, Q&A: 10

Factors implicated in the pathophysiology of intestinal inflammation include defects in intestinal epithelial barrier function, abnormal immune responses, and activities of the gut microbiota. Current agents used to treat human Inflammatory Bowels ...

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Hello
I think I did not ask my questions well. The pellet from the ultracentrifugation step (isolation step) was dissolved in PBS (as shown). Should this solution be clear? Or should this concentration exist? And is the purification step necessary? Or can the same solution be used for in vitro experiments?

1/15/2021 8:41:18 AM Reply

Chunhua Yang

Institute for Biomedical Science, Georgia state university, United States

This solution is looking good. If you used sterile PBS, then it can be used in in vitro experiments.

2021-01-17 19:33:41

Junsik Sung

Institute for Biomedical Science, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA

Dissolved pellets in PBS shouldn't be looking clear. Purification process is an important step for later use.

2021-01-17 18:05:58

In vitro Intestinal Epithelial Wound-healing Assays Using Electric Cell-Substrate Impedance Sensing Instrument

Authors: Olivier Merlin-Zhang, Junsik Sung and Emilie Viennois, date: 09/05/2019, view: 1679, Q&A: 0

Here, we describe an in vitro epithelial wound-healing assay using Electric Cell-Substrate Impedance Sensing (ECIS) technology. The ECIS technology is a real time cell growth assay based on a small (250 μm diameter) active gold electrode ...

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4 Protocols reviewed

Attachment of a ³²P-phosphate to the 3′ Terminus of a DNA Oligonucleotide

Authors: Joshua C. Cofsky and Jennifer A. Doudna, date: 10/20/2020, view: 920, Q&A: 0

Biochemical investigations into DNA-binding and DNA-cutting proteins often benefit from the specific attachment of a radioactive label to one of the two DNA termini. In many cases, it is essential to perform two versions of the same experiment: one ...

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Glioma Induction by Intracerebral Retrovirus Injection

Authors: Ravinder K Verma , Fanghui Lu and Qing Richard Lu, date: 07/20/2017, view: 6622, Q&A: 0

Glioblastoma (GBM) is the most common primary brain cancer in adults and has a poor prognosis. It is characterized by a high degree of cellular infiltration that leads to tumor recurrence, atypical hyperplasia, necrosis, and angiogenesis. Despite ...

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