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A peer-reviewed protocol journal. No publication fee; no access fee.
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Reviewed
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Didier Merlin
Institute for Biomedical Science, Georgia state university, United States
4 protocols
Chunhua Yang
Institute for Biomedical Science, Georgia state university, United States
3 protocols
Junsik Sung
Institute for Biomedical Science, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA
4 protocols
Mingzhen Zhang
Georgia State University, USA
2 protocols
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Ana Almeida
University College Cork
7 protocols
Andrea Puhar
The Laboratory For Molecular Infection Medicine Sweden (MIMS) and Department of Molecular Biology, Umeå University, Sweden
48 protocols
Anna Zorina
Russian Academy of Sciences
5 protocols
Kathrin Sutter
University of Duisburg-Essen
15 protocols
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Reviewer
Emilie Viennois
Assistant Professor, INSERM
Research focus
Molecular biology
Inflammatory Bowel diseases
Cancer
Microbiota
Contributions
3
Author merit
Reviewer merit
Editor merit
Education
PhD, Universite Blaise Pascal, France, 2011
Publications
https://scholar.google.com/citations?user=byweUwIAAAAJ&hl=en
3 Protocols published
Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of
Zingiber officinale
Authors:
Junsik Sung
,
Chunhua Yang
,
Emilie Viennois
,
Mingzhen Zhang
and
Didier Merlin
,
date:
10/05/2019,
view:
2579,
Q&A:
1
Factors implicated in the pathophysiology of intestinal inflammation include defects in intestinal epithelial barrier function, abnormal immune responses, and activities of the gut microbiota. Current agents used to treat human Inflammatory Bowels ...
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Variya Nemidkanam
Chulalongkorn University
Thank you for the helpful protocol.
I already follow your protocol but I facing the sterility problem.
How do you dealing with bacterial contamination in the isolated vesicle?
Best regards,
11/10/2019 4:54:40 AM
Reply
Junsik Sung
Institute for Biomedical Science, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA
Hi Variya,
In order to avoid contamination, all the agents should be sterilized and filtrate with 0.22 um filter. After suspension, it needs to be sterilized under UV light for about 30 min before you use it for in vitro and in vivo experiment. I hope this helps for your work. Please let me know if you have any other questions on the protocol. Thank you.
2019-11-11 12:22:45
In vitro
Intestinal Epithelial Wound-healing Assays Using Electric Cell-Substrate Impedance Sensing Instrument
Authors:
Olivier Merlin-Zhang
,
Junsik Sung
and
Emilie Viennois
,
date:
09/05/2019,
view:
1537,
Q&A:
0
Here, we describe an
in vitro
epithelial wound-healing assay using Electric Cell-Substrate Impedance Sensing (ECIS) technology. The ECIS technology is a real time cell growth assay based on a small (250 μm diameter) active gold electrode ...
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4 Protocols reviewed
Attachment of a
32
P-phosphate to the 3′ Terminus of a DNA Oligonucleotide
Authors:
Joshua C. Cofsky
and
Jennifer A. Doudna
,
date:
10/20/2020,
view:
380,
Q&A:
0
Biochemical investigations into DNA-binding and DNA-cutting proteins often benefit from the specific attachment of a radioactive label to one of the two DNA termini. In many cases, it is essential to perform two versions of the same experiment: one ...
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Glioma Induction by Intracerebral Retrovirus Injection
Authors:
Ravinder K Verma
,
Fanghui Lu
and
Qing Richard Lu
,
date:
07/20/2017,
view:
6362,
Q&A:
0
Glioblastoma (GBM) is the most common primary brain cancer in adults and has a poor prognosis. It is characterized by a high degree of cellular infiltration that leads to tumor recurrence, atypical hyperplasia, necrosis, and angiogenesis. Despite ...
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