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A peer-reviewed protocol journal. No publication fee; no access fee.
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Coreviewers
Alka Mehra
Institute of Genomics and Integrative Biology
18 protocols
Andrea Puhar
MIMS - Umea University
29 protocols
Meenal Sinha
University of California
12 protocols
Ralph Boettcher
Max Planck Institute for Biochemistry
29 protocols
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Reviewer
Saskia F. Erttmann
Post-Doc, Umeå University
Research focus
Immunology
Signal transduction, host-microbial interaction, innate immunity
Contributions
Reviewer merit
Editor merit
Education
PhD, University of Greifswald, Germany, 2011
Lab information
Nelson O. Gekara Lab
4 Protocols reviewed
Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation
Authors:
Emilie Viennois
,
Anika Tahsin
and
Didier Merlin
,
date:
05/05/2018,
view:
382,
Q&A:
0
We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium ...
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Detection of ASC Oligomerization by Western Blotting
Authors:
Jérôme Lugrin
and
Fabio Martinon
,
date:
05/20/2017,
view:
3622,
Q&A:
0
The apoptosis-associated speck-like protein with a caspase-recruitment domain (ASC) adaptor protein bridges inflammasome sensors and caspase-1. Upon inflammasome activation, ASC nucleates in a prion-like manner into a large and single platform ...
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