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A peer-reviewed protocol journal. No publication fee; no access fee.
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Coreviewers
Alka Mehra
Institute of Genomics and Integrative Biology
19 protocols
Andrea Puhar
MIMS - Umeå University
36 protocols
Meenal Sinha
University of California
13 protocols
Ralph Boettcher
Max Planck Institute for Biochemistry
33 protocols
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Reviewer
Saskia F. Erttmann
Post-Doc, Umeå University
Research focus
Immunology
Signal transduction, host-microbial interaction, innate immunity
Contributions
Reviewer merit
Editor merit
Education
PhD, University of Greifswald, Germany, 2011
Lab information
Nelson O. Gekara Lab
5 Protocols reviewed
A Highly Sensitive Anion Exchange Chromatography Method for Measuring cGAS Activity
in vitro
Authors:
Andreas Holleufer
and
Rune Hartmann
,
date:
10/20/2018,
view:
97,
Q&A:
0
Cyclic GMP-AMP synthase (cGAS) is a pattern recognition receptor (PRR) that senses double stranded DNA (dsDNA) in the cytosol and this leads to the activation of stimulator of interferon genes (STING) via the secondary messenger 2’3’-cyclic GMP-AMP ...
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Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation
Authors:
Emilie Viennois
,
Anika Tahsin
and
Didier Merlin
,
date:
05/05/2018,
view:
1130,
Q&A:
0
We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium ...
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