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Coauthors
Magdalena Mosiolek Gregor Mendel Institute, Austrian Academy of Sciences, Austria
1 protocol

Nina Chumak Gregor Mendel Institute, Austrian Academy of Sciences, Austria
1 protocol
Coreviewers
Agnieszka Zienkiewicz Georg August University
12 protocols

Dennis Nürnberg Imperial College London
31 protocols

Jihyun Kim Finless Foods
18 protocols

Reviewer
Vera Karolina Schoft
  • Viehmarktgasse 2a
Research focus
  • Plant science
  • 1 Author merit

Education

Ph.D. in Genetics, Max F. Perutz Laboratories, University of Vienna, Austria, 2005

Current position

Staff Scientist, Campus Science Support Facilities GmbH (CSF), Vienna, Austria

Publications

  1. Schoft, V. K., Chumak, N., Bindics, J., Slusarz, L., Twell, D., Köhler, C. and Tamaru, H. (2015). SYBR Green-activated sorting of Arabidopsis pollen nuclei based on different DNA/RNA content. Plant Reprod 28(1): 61-72.
  2. Mérai, Z., Chumak, N., García-Aguilar, M., Hsieh, T. F., Nishimura, T., Schoft, V. K., Bindics, J., Slusarz, L., Arnoux, S., Opravil, S., Mechtler, K., Zilberman, D., Fischer, R. L. and Tamaru, H. (2014). The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes. Proc Natl Acad Sci U S A 111(45): 16166-16171.
  3. Schatlowski, N., Wolff, P., Santos-González, J., Schoft, V., Siretskiy, A., Scott, R., Tamaru, H. and Köhler, C. (2014). Hypomethylated pollen bypasses the interploidy hybridization barrier in Arabidopsis. Plant Cell 26(9): 3556-3568.
  4. Ibarra, C. A.*, Feng, X.*, Schoft, V. K.*, Hsieh, T. F.*, Uzawa, R., Rodrigues, J. A., Zemach, A., Chumak, N., Machlicova, A., Nishimura, T., Rojas, D., Fischer, R. L., Tamaru, H. and Zilberman, D. (2012). Active DNA demethylation in plant companion cells reinforces transposon methylation in gametes. Science 337(6100): 1360-1364.
  5. Schoft, V. K., Chumak, N., Choi, Y., Hannon, M., Garcia-Aguilar, M., Machlicova, A., Slusarz, L., Mosiolek, M., Park, J. S., Park, G. T., Fischer, R. L. and Tamaru, H. (2011). Function of the DEMETER DNA glycosylase in the Arabidopsis thaliana male gametophyte. Proc Natl Acad Sci U S A 108(19): 8042-8047.
  6. Schoft, V. K., Chumak, N., Mosiolek, M., Slusarz, L., Komnenovic, V., Brownfield, L., Twell, D., Kakutani, T. and Tamaru, H. (2009). Induction of RNA-directed DNA methylation upon decondensation of constitutive heterochromatin. EMBO Rep 10(9): 1015-1021.
  7. Schoft, V. K., Schopoff, S. and Jantsch, M. F. (2007). Regulation of glutamate receptor B pre-mRNA splicing by RNA editing. Nucleic Acids Res 35(11): 3723-3732.
  8. Hitti, E. G., Sallacz, N. B., Schoft, V. K. and Jantsch, M. F. (2004). Oligomerization activity of a double-stranded RNA-binding domain. FEBS Lett 574(1-3): 25-30.
  9. Schoft, V. K.*, Beauvais, A. J.*, Lang, C., Gajewski, A., Prüfert, K., Winkler, C., Akimenko, M. A., Paulin-Levasseur, M. and Krohne, G. (2003). The lamina-associated polypeptide 2 (LAP2) isoforms beta, gamma and omega of zebrafish: developmental expression and behavior during the cell cycle. J Cell Sci 116(Pt 12): 2505-2517.
1 Protocol published
Sample Preparation and Fractionation of Arabidopsis thaliana Sperm and Vegetative Cell Nuclei by FACS
Authors:  Nina Chumak, Magdalena Mosiolek and Vera Karolina Schoft, date: 11/20/2015, view: 3863, Q&A: 0
One of the major topics in plant and animal biology is sexual reproduction. It is, therefore, of great interest to isolate and study germ cells and accessory cells. The male gametophyte of the flowering plant Arabidopsis thaliana (A. ...
2 Protocols reviewed
CRISPR/Cas9 Gene Editing in the Marine Diatom Phaeodactylum tricornutum
The establishment of the CRISPR/Cas9 technology in diatoms (Hopes et al., 2016; Nymark et al., 2016) enables a simple, inexpensive and effective way of introducing targeted alterations in the genomic DNA of this highly important ...
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Improving CRISPR Gene Editing Efficiency by Proximal dCas9 Targeting
Authors:  Fuqiang Chen, Xiao Ding, Yongmei Feng, Timothy Seebeck, Yanfang Jiang and Gregory D Davis, date: 08/05/2017, view: 1447, Q&A: 0
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems function as an adaptive immune system in bacteria and archaea for defense against invading viruses and plasmids (Barrangou and Marraffini, 2014). ...
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