Francisca Mulero Molecular Imaging Unit, Biotechnology Programme, Centro Nacional de Investigaciones Oncológicas (CNIO), Spain
1 protocol

David Santamaría Experimental Oncology, Molecular Oncology Programme, Centro Nacional de Investigaciones Oncológicas (CNIO), Spain
1 protocol

Patricia Nieto Experimental Oncology, Molecular Oncology Programme, Centro Nacional de Investigaciones Oncológicas (CNIO), Spain
1 protocol

Juan Cámara Molecular Imaging Unit, Biotechnology Programme, Centro Nacional de Investigaciones Oncológicas (CNIO), Spain
1 protocol
HongLok Lung University of Hong Kong
58 protocols

Chiara Ambrogio
  • Research scientist, Dana Farber Cancer Institute
Research focus
  • Cancer biology
  • Lung cancer
  • 1 Author merit


PhD, University of Torino, 2008

Lab information

Pasi Jänne's lab


1) Ambrogio C, Voena C, Manazza AD, Piva R, Riera L, Barberis L, Costa C, Tarone G, Defilippi P, Hirsch E, Erba EB, Mohammed S, Jensen ON, Palestro G, Inghirami G, Chiarle R. p130Cas mediates the transforming properties of the anaplastic lymphoma kinase. Blood. 2005 Dec 1;106(12):3907-16.
2) Piva R, Chiarle R, Manazza AD, Taulli R, Simmons W, Ambrogio C, D'Escamard V, Pellegrino E, Ponzetto C, Palestro G, Inghirami G. Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas. Blood. 2006 Jan 15;107(2):689-97.
3) Voena C, Conte C, Ambrogio C, Boeri Erba E, Boccalatte F, Mohammed S, Jensen ON, Palestro G, Inghirami G, Chiarle R. The tyrosine phosphatase Shp2 interacts with NPM-ALK and regulates anaplastic lymphoma cell growth and migration. Cancer Res. 2007 May 1;67(9):4278-86.
4) Berto G, Camera P, Fusco C, Imarisio S, Ambrogio C, Chiarle R, Silengo L, Di Cunto F. The Down syndrome critical region protein TTC3 inhibits neuronal differentiation via RhoA and Citron kinase. J Cell Sci. 2007 Jun 1;120(Pt 11):1859-67.
5) Costa C, Barberis L, Ambrogio C, Manazza AD, Patrucco E, Azzolino O, Nielssen P, Circolo E, Altruda F, Prestwich G, Chiarle R, Wymann M, Ridley A, Hirsch E. Constitutive elevation of phosphoinositide 3-Kinase γ activity in mice does not increase tumor susceptibility but reveals a negative feedback loop regulating Rac during leukocyte chemotaxis. PNAS 2007 Sept 4; 104(36):14354-9.
6) Chiarle R, Voena C, Ambrogio C, Piva R, Inghirami G. The anaplastic lymphoma kinase in the pathogenesis of cancer. Nat Rev Cancer. 2008 Jan;8(1):11-23.
7) Chiarle R, Martinengo C, Mastini C, Ambrogio C, D’Escamard V, Forni G, Inghirami G. Anaplastic Lymphoma Kinase is an effective oncoantigen for lymphoma vaccination. Nat Med 2008 Jun; 14(6): 676-80.
8) Crosetto N, Bienko M, Hibbert RG, Perica T, Ambrogio C, Kensche T, Hofmann K, Sixma TK, Dikic I. Human WRNIP1 is localized in replication factories in a UBZ-dependent manner. J Biol Chem. 2008 Dec 12;283(50):35173-85.
9) Ambrogio C, Voena C, Manazza AD, Martinengo C, Costa C, Kirchhausen T, Hirsch E, Inghirami G and Chiarle R. Anaplastic Lymphoma Kinase controls cell shape and growth of Anaplastic Large Cell Lymphoma through Cdc42. Cancer Res 2008 Nov 1;68(21):8899-907.
10) Barberis L, Pasquali C, Bertschy-Meier D, Cuccurullo A, Costa C, Ambrogio C, Vilbois F, Chiarle R, Wymann M, Altruda F, Rommel C, Hirsch E. Leukocyte transmigration is modulated by chemokine-mediated, PI3Kγ-dependent phosphorylation of vimentin. European Journal of Immunology 2009 Apr;39(4):1136-46.
11) Gonzalo P, Guadamillas MC, Hernández-Riquer MV, Pollán A, Grande-García A, Bartolomé RA, Vasanji A, Ambrogio C, Chiarle R, Teixidó J, Risteli J, Apte SS, del Pozo MS, and Arroyo AG. Membrane type 1-matrix metalloproteinase contributes to macrophage fusion through the regulation of the Rac1 signaling pathway. Dev Cell. 2010 Jan 19;18(1):77-89.
12) Ambrogio C, Martinengo C, Voena C, Tondat F, Francia di Celle P, Inghirami G and Chiarle R. NPM-ALK oncogenic tyrosine kinase controls T cell identity by transcriptional and epigenetic silencing in lymphoma cells. Cancer Res 2009 Nov 15;69(22):8611-9.
13) Riera L, Lasorsa E, Ambrogio C, Surrenti N, Voena C, Chiarle R. Involvement of Grb2 adaptor protein in nucleophosmin-anaplastic lymphoma kinase (NPM-ALK)-mediated signaling and anaplastic large cell lymphoma growth. J Biol Chem. 2010 Aug 20;285(34):26441-50.
14) Boselli D, Ragimbeau J, Orlando L, Cappello P, Capello M, Ambrogio C, Chiarle R, Marsili G, Battistini A, Giovarelli M, Pellegrini S, Novelli F. Expression of IFNgammaR2 mutated in a dileucine internalization motif reinstates IFNgamma signaling and apoptosis in human T lymphocytes. Immunol Lett. 2010 2010 Nov 30;134(1):17-25.
15) Di Paolo D*, Ambrogio C*, Pastorino F, Brignole C, Martinengo C, Carosio R, Loi M, Pagnan G, Emionite L, Cilli M, Ribatti D, Allen TM, Chiarle R, Ponzoni M, Perri P. Selective Therapeutic Targeting of the Anaplastic Lymphoma Kinase With Liposomal siRNA Induces Apoptosis and Inhibits Angiogenesis in Neuroblastoma. Mol Ther. 2011 Aug 9. doi: 10.1038/mt.2011.142.
*equally contributed.
16) Bosio Y, Berto G, Camera P, Bianchi F, Ambrogio C, Claus P, Di Cunto F. PPP4R2 regulates neuronal cell differentiation and survival, functionally cooperating with SMN. Eur J Cell Biol. 2012 Aug;91(8):662-74. Epub 2012 May 3.
17) Voena C, Di Giacomo F, Panizza E, D'Amico L, Boccalatte FE, Pellegrino E, Todaro M, Recupero D, Tabbò F, Ambrogio C, Martinengo C, Bonello L, Pulito R, Hamm J, Chiarle R, Cheng M, Ruggeri B, Medico E, Inghirami G. The EGFR family members sustain the neoplastic phenotype of ALK+ lung adenocarcinoma via EGR1. Oncogenesis. 2013 Apr 8;2:e43. doi: 10.1038/oncsis.2013.7.
18) Piazza R, Magistroni V, Mogavero A, Andreoni F, Ambrogio C, Chiarle R, Mologni L, Bachmann PS, Lock RB, Collini P, Pelosi G, Gambacorti-Passerini C. Epigenetic silencing of the proapoptotic gene BIM in anaplastic large cell lymphoma through an MeCP2/SIN3a deacetylating complex. Neoplasia. 2013 May;15(5):511-22.
19) Martinengo C, Poggio T, Menotti M, Scalzo MS, Mastini C, Ambrogio C, Pellegrino E, Riera L, Piva R, Ribatti D, Pastorino F, Perri P, Ponzoni M, Wang Q, Voena C, Chiarle R. ALK-Dependent Control of Hypoxia-Inducible Factors Mediates Tumor Growth and Metastasis. Cancer Res. 2014 Sep 5. [Epub ahead of print].
20) Ambrogio C, Carmona FJ, Vidal A, Falcone M, Nieto P, Romero OA, Puertas S, Vizoso M, Nadal E, Poggio T, Sánchez-Céspedes M, Esteller M, Mulero F, Voena C, Chiarle R, Barbacid M, Santamaría D, Villanueva A. Modeling Lung Cancer Evolution and Preclinical Response by Orthotopic Mouse Allografts. Cancer Res. 2014 Sep 12. [Epub ahead of print].
21) Ambrogio C, Stern P, Scuoppo C, Kranz H, Barbacid M, Santamaría D. Lentiviral-based approach for the validation of cancer therapeutic targets in vivo. Biotechniques. 2014 Oct 1;57(4):179-87. doi: 10.2144/000114214. eCollection 2014.
22) Blasco RB, Karaca E, Ambrogio C, Cheong TC, Karayol E, Minero VG, Voena C, Chiarle R. Simple and rapid in vivo generation of chromosomal rearrangements using CRISPR/Cas9 technology. Cell Rep. 2014 Nov 20;9(4):1219-27. doi: 10.1016/j.celrep.2014.10.051. Epub 2014 Nov 13.
23) Lopez-Contreras AJ, Specks J, Barlow JH, Ambrogio C, Desler C, Vikingsson S, Rodrigo-Perez S, Green H, Rasmussen LJ, Murga M, Nussenzweig A, Fernandez-Capetillo O. Increased Rrm2 gene dosage reduces fragile site breakage and prolongs survival of ATR mutant mice. Genes Dev. 2015 Apr 1;29(7):690-5. doi: 10.1101/gad.256958.114.
24) García-Beccaria M, Martínez P, Méndez-Pertuz M, Martínez S, Blanco-Aparicio C, Cañamero M, Mulero F, Ambrogio C, Flores JM, Megias D, Barbacid M, Pastor J, Blasco MA. Therapeutic inhibition of TRF1 impairs the growth of p53-deficient K-RasG12V-induced lung cancer by induction of telomeric DNA damage. EMBO Mol Med. 2015 May 13;7(7):930-49.
25) Voena C, Menotti M, Mastini C, Di Giacomo F, Longo DL, Castella B, Merlo ME, Ambrogio C, Wang Q, Minero VG, Poggio T, Martinengo C, D'Amico L, Panizza E, Mologni L, Cavallo F, Altruda F, Butaney M, Capelletti M, Inghirami G, Jänne PA, Chiarle R. Efficacy of a Cancer Vaccine against ALK-Rearranged Lung Tumors. Cancer Immunol Res. 2015 Sep 29.
26) Ambrogio C, Cámara JA, Nieto P, Santamaría D, Mulero F. Analysis of Murine Lung Tumors by Micro PET-CT Imaging. Bio-protocol, Vol 5, Issue 24, 2015 December.
27) Ceccon M, Merlo ME, Mologni L, Poggio T, Varesio LM, Menotti M, Bombelli S, Rigolio R, Manazza AD, Di Giacomo F, Ambrogio C, Giudici G, Casati C, Mastini C, Compagno M, Turner SD, Gambacorti-Passerini C, Chiarle R, Voena C. Excess of NPM-ALK oncogenic signaling promotes cellular apoptosis and drug dependency. Oncogene. 2016 Jul 21;35(29):3854-65. doi: 10.1038/onc.2015.456.
28) Choudhari R, Minero VG, Menotti M, Pulito R, Brakebusch C, Compagno M, Voena C, Ambrogio C*, Chiarle R*. Non redundant roles for Cdc42 and Rac1 in ALK-rearranged lymphoma. Blood. 2016 Jan 8. pii: blood-2015-11-683052. *Co-last name.
29) Ambrogio C*, Gómez-López G, Falcone M, Villanueva A, Crosetto N, Blasco R, Sánchez-Céspedes M, Ren X, Wang Z, Ding K, Serrano M, Hidalgo M, Santamaría D*, Barbacid M*. Combined inhibition of Ddr1 and Notch signaling as an effective therapeutic strategy for K-Ras-driven/p53-null lung adenocarcinomas. Nat Med. 2016 Feb 8. doi: 10.1038/nm.4041. *Co-corresponding author.
(Referred by Research Watch in Cancer Discovery: Dual Therapy with DDR1 and Notch Inhibitors Kills KRAS-Mutant Lung Tumors. Cancer Discov. 2016 Feb 18.)
30) Ambrogio C*, Nadal E, Villanueva A, Gómez-López G, Cash TP, Barbacid M, Santamaría D. KRAS-driven lung adenocarcinoma: combined DDR1/Notch inhibition as an effective therapy. ESMO Open. 2016 Sept 1. *Corresponding author.
31) Ambrogio C*, Barbacid M, Santamaría D*. In vivo oncogenic conflict triggered by co-existing KRAS and EGFR activating mutations in lung adenocarcinoma. Oncogene. 2016 Oct 24. *Co-corresponding author.
32) Compagno M, Wang Q, Pighi C, Cheong TC, Meng FL, Poggio T, Yeap LS, Karaca E, Blasco RB, Langellotto F, Ambrogio C, Voena C, Wiestner A, Kasar SN, Brown JR, Sun J, Wu CJ, Gostissa M, Alt FW, Chiarle R. Phosphatidylinositol 3-kinase δ blockade increases genomic instability in B cells. Nature. 2017 Feb 23;542(7642):489-493. doi: 10.1038/nature21406. Epub 2017 Feb 15.
33) Kohler J, Catalano M, Ambrogio C. Back to the bench? MEK and ERK inhibitors for the treatment of KRAS mutant lung adenocarcinoma. Curr Med Chemistry. 2017 May 29. doi: 10.2174/0929867324666170530093100. [Epub ahead of print].
34) Nieto P, Ambrogio C, de Esteban L, Gómez-López G, Blasco MT, Yao Z, Marais R, Rosen N, Chiarle R, Pisano DG, Barbacid M, Santamaría D. A B-Raf kinase inactive mutant induces lung adenocarcinoma. Nature (in press).
35) Zeng M, Lu J, Li L, Feru F, Quan C, Gero TW, Ficarro SB, Xiong Y, Ambrogio C, Paranal RM, Catalano M, Shao J, Wong KK, Fischer ES, Marto JA, Janne PA, Scott DA, Westover KD, Gray NS. Potent and Selective Covalent Quinazoline Inhibitors of KRAS G12C. Cell Chemical Biol (in press).
1 Protocol published
Analysis of Murine Lung Tumors by Micro PET-CT Imaging
Accurate live tumor imaging in mice is now possible by means of high-resolution positron emission tomography (micro-PET) and X-ray computed tomography (micro-CT). By providing a powerful tool to examine biological samples with complex structure in ...
2 Protocols reviewed
Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets
Authors:  Pornima Phatak and James M Donahue, date: 05/05/2017, view: 4357, Q&A: 1
microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.
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In situ Hybridization (ISH) and Quantum Dots (QD) of miRNAs
Authors:  Sajni Josson, Murali Gururajan and Leland W.K. Chung, date: 02/20/2017, view: 3879, Q&A: 0
miRNA are short non-coding RNA which inhibit translation of mRNA. miRNA regulate several cellular processes. Certain miRNA are known to induce oncogenesis. miRNA can be measured by real-time PCR and be imaged using a combination of in situ ...
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1 Protocol edited
3D Culture Protocol for Testing Gene Knockdown Efficiency and Cell Line Derivation
Authors:  Jan Strnadel, Sang Myung Woo, Sunkyu Choi, Huawei Wang, Marian Grendar and Ken Fujimura, date: 06/05/2018, view: 1363, Q&A: 0
Traditional 2D cell cultures with cells grown as monolayers on solid surface still represent the standard method in cancer research for drug testing. Cells grown in 2D cultures, however, lack relevant cell-matrix and cell-cell interactions and ...
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