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A peer-reviewed protocol journal. No publication fee; no access fee.
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Alba Blesa
Universidad Francisco de Vitoria
4 protocols
Amit Dey
Regional Centre for Biotechnology
10 protocols
Dennis Nürnberg
Imperial College London
38 protocols
Fanglian He
Bio-protocol
205 protocols
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Reviewer
Seda Ekici
University of Washington
Research focus
Microbiology
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7 Protocols reviewed
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Authors:
Ana Lechuga
,
Mónica Berjón-Otero
,
Margarita Salas
and
Modesto Redrejo-Rodríguez
,
date:
01/05/2018,
view:
1802,
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This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in
E. coli
as HA-tagged recombinant protein and cell-free extracts are ...
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Evaluation of Plasmid Stability by Negative Selection in Gram-negative Bacteria
Authors:
Damián Lobato Márquez
and
Laura Molina García
,
date:
05/05/2017,
view:
3678,
Q&A:
0
Plasmid stability can be measured using antibiotic-resistance plasmid derivatives by positive selection. However, highly stable plasmids are below the sensitivity range of these assays. To solve this problem we describe a novel, highly sensitive ...
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