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A peer-reviewed protocol journal. No publication fee; no access fee.
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Alba Blesa
Universidad Francisco de Vitoria
23 protocols
Amit Dey
International Centre for Genetic Engineering and Biotechnology
17 protocols
Dennis Nürnberg
Freie Universität Berlin
48 protocols
Fanglian He
Bio-protocol
205 protocols
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Reviewer
Seda Ekici
University of Washington
Research focus
Microbiology
Contributions
Reviewer merit
Editor merit
8 Protocols reviewed
Measurement of ATPase Activity of Valosin-containing Protein/p97
Authors:
Kruthi Suvarna
,
Kaori Honda
,
Makoto Muroi
,
Yasumitsu Kondoh
,
Hiroyuki Osada
and
Nobumoto Watanabe
,
date:
02/05/2020,
view:
2066,
Q&A:
0
Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was ...
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Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Authors:
Ana Lechuga
,
Mónica Berjón-Otero
,
Margarita Salas
and
Modesto Redrejo-Rodríguez
,
date:
01/05/2018,
view:
6511,
Q&A:
0
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in
E. coli
as HA-tagged recombinant protein and cell-free extracts are ...
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