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Alba Blesa Universidad Francisco de Vitoria
 18 protocols

Amit Dey International Centre for Genetic Engineering and Biotechnology
 14 protocols

Dennis Nürnberg Imperial College London
 48 protocols

Fanglian He Bio-protocol
 205 protocols

Reviewer
Seda Ekici
  • University of Washington
Research focus
  • Microbiology
8 Protocols reviewed
Measurement of ATPase Activity of Valosin-containing Protein/p97
Authors:  Kruthi Suvarna, Kaori Honda, Makoto Muroi, Yasumitsu Kondoh, Hiroyuki Osada and Nobumoto Watanabe, date: 02/05/2020, view: 1668, Q&A: 0
Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was ...
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Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Authors:  Ana Lechuga, Mónica Berjón-Otero, Margarita Salas and Modesto Redrejo-Rodríguez, date: 01/05/2018, view: 6062, Q&A: 0
This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are ...
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