Kristin L. Shingler —BIO-PROTOCOL

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Susan Hafenstein Department of Medicine, Pennsylvania State University College of Medicine, USA
1 protocol

Lindsey Organtini Microbiology and Immunology Department, Pennsylvania State University College of Medicine, USA
1 protocol

Alba Blesa Universidad Francisco de Vitoria
12 protocols

Angela Corona University of Cagliari
3 protocols

Balaji Olety Amaranath University of Massachusetts medical School
2 protocols

Beatrice Li University of Oxford
4 protocols

Editor

Kristin L. Shingler

Teaching Specialist, University of Minnesota School of Dentistry

Research focus

Microbiology
bacteriophage, DNA packaging, molecular motors, Phi29

1 Author merit
Reviewer merit
Editor merit

Education

PhD, Penn State College of Medicine, 2015

Lab information

Paul Jardine Lab

Publications

https://www.ncbi.nlm.nih.gov/sites/myncbi/kristin.shingler.1/bibliography/57140528/public/?sort=date&direction=ascending

1 Protocol published

Enterovirus 71 Virus Propagation and Purification

Authors: Kristin L. Shingler, Lindsey J. Organtini and Susan Hafenstein, date: 05/05/2014, view: 10063, Q&A: 0

Since its discovery in 1969, enterovirus 71 (EV71) has emerged as a serious worldwide health threat. This member of the picornavirus family causes hand, foot, and mouth disease, and also has the capacity to invade the central nervous system to cause ...

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12 Protocols reviewed

Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria

Authors: Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias and Elena Mercadé, date: 09/20/2019, view: 622, Q&A: 0

A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so ...

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On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates

Authors: Roland Remenyi, Raymond Li and Mark Harris, date: 02/20/2019, view: 2132, Q&A: 0

Advanced labeling technologies allow researchers to study protein turnover inside intact cells and to track the labeled protein in downstream applications. In the context of a viral infection, the combination of imaging and fluorescent labeling of ...

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5 Protocols edited

Study of Microbial Extracellular Vesicles: Separation by Density Gradients, Protection Assays and Labelling for Live Tracking

Authors: Carolina Coelho, Raghav Vij, Daniel Q. Smith, Nathan R. Brady, Anne Hamacher-Brady and Arturo Casadevall, date: 01/20/2020, view: 167, Q&A: 0

Extracellular vesicles (EVs) are produced by all domains of life including Bacteria, Archaea and Eukarya. EVs are critical for cellular physiology and contain varied cargo: virulence factors, cell wall remodeling enzymes, extracellular matrix ...

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Bacterial Synchronized Transfer Assays in Bone Marrow Derived Macrophages

Authors: Sedelia R Dominguez, Tom Kawula and Shaun P Steele, date: 11/20/2019, view: 507, Q&A: 0

Merocytophagy (“mero”, Greek for partial; “cytophagy” for cell eating) is a process by which cells acquire microbes and cytosolic material through phagocytosis of a small portion of neighboring cells upon cell-cell contact. Cell-cell contact ...

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