Kristin L. Shingler —BIO-PROTOCOL

Home About Us

Archive

Susan Hafenstein Department of Medicine, Pennsylvania State University College of Medicine, USA
1 protocol

Lindsey Organtini Microbiology and Immunology Department, Pennsylvania State University College of Medicine, USA
1 protocol

Alba Blesa Universidad Francisco de Vitoria
18 protocols

Angela Corona University of Cagliari
3 protocols

Ayush Ranawade harvard university
4 protocols

Balaji Olety Amaranath University of Massachusetts medical School
3 protocols

Editor

Kristin L. Shingler

Teaching Specialist, University of Minnesota School of Dentistry

Research focus

Microbiology
bacteriophage, DNA packaging, molecular motors, Phi29

1 Author merit
Reviewer merit
Editor merit

Education

PhD, Penn State College of Medicine, 2015

Lab information

Paul Jardine Lab

Publications

https://www.ncbi.nlm.nih.gov/sites/myncbi/kristin.shingler.1/bibliography/57140528/public/?sort=date&direction=ascending

1 Protocol published

Enterovirus 71 Virus Propagation and Purification

Authors: Kristin L. Shingler, Lindsey J. Organtini and Susan Hafenstein, date: 05/05/2014, view: 11074, Q&A: 0

Since its discovery in 1969, enterovirus 71 (EV71) has emerged as a serious worldwide health threat. This member of the picornavirus family causes hand, foot, and mouth disease, and also has the capacity to invade the central nervous system to cause ...

More >

12 Protocols reviewed

Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria

Authors: Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias and Elena Mercadé, date: 09/20/2019, view: 1788, Q&A: 0

A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so ...

More >

On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates

Authors: Roland Remenyi, Raymond Li and Mark Harris, date: 02/20/2019, view: 3070, Q&A: 0

Advanced labeling technologies allow researchers to study protein turnover inside intact cells and to track the labeled protein in downstream applications. In the context of a viral infection, the combination of imaging and fluorescent labeling of ...

More >

More protocols >

12 Protocols edited

Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells

Authors: Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd, date: 09/05/2020, view: 976, Q&A: 0

Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate ...

More >

Candida albicans Agar Invasion Assays

Authors: Shamoon Naseem, Lois M. Douglas and James B. Konopka, date: 08/20/2020, view: 638, Q&A: 0

The ability of the human fungal pathogen Candida albicans to disseminate into tissues is promoted by a switch from budding to invasive hyphal growth. This morphological transition is stimulated by multiple environmental factors that can ...

More >

More protocols >