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Coauthors
Justo Castaño Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols

Raúl Luque Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols
Coreviewers
Daniel Kraus Würzburg University Hospital
6 protocols

Gal Haimovich Weizmann Institute of Science
26 protocols

Kae-Jiun Chang University of California, San Francisco
11 protocols

Oneil Bhalala Baker Institute
32 protocols

Reviewer
Manuel D Gahete
  • Post-Doc, IMIBIC - Maimonides Institute for Biomedical Research of Córdoba
Research focus
  • Cancer biology
  • 2 Author merit

Education

PhD, University of Cordoba, 2010

2 Protocols published
Measurement of Free Cytosolic Calcium Concentration ([Ca2+]i) in Single CHO-K1 Cells
Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 6624, Q&A: 0
This is a protocol to analyze the functional response of single CHO-K1 cells to a given treatment in terms of changes in free cytosolic calcium concentration ([Ca2+]i). This is possible by using the Ca2+ indicator dye Fura-2 AM, a polyamino ...
GPCRs Interaction Measurement by Fluorescence Resonance Energy Transfer (FRET)
Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 4894, Q&A: 0
This is a protocol to determine the physical interaction of a G-protein coupled receptor (GPCR) with itself (homodimerization) or with other GPCR (heterodimerazation) using fluorescence resonance energy transfer (FRET). FRET is a distance-dependent ...
5 Protocols reviewed
FRET-based Stoichiometry Measurements of Protein Complexes in vitro
Authors:  Francesca Mattiroli, Yajie Gu and Karolin Luger, date: 02/05/2018, view: 182, Q&A: 0
For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry ...
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Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices
Authors:  Jillian M. Daniel, Jim Deuchars and Susan A. Deuchars, date: 10/05/2016, view: 2761, Q&A: 0
In these culture models, the normal cytoarchitecture and local neuronal circuits of the spinal cord are preserved, offering a compromise between dissociated cell cultures and complete animal studies. The addition of 5-ethynyl-2’-deoxyuridine (EdU) ...
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