Justo Castaño Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols

Raúl Luque Department of Cell Biology, Physiology and Immunology, University of Córdoba, Reina Sofía University Hospital, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), and CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Spain
2 protocols
Daniel Kraus Mainz University Hospital
6 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
93 protocols

Imre Gáspár INSTITUTE OF MOLECULAR BIOTECHNOLOGY, IMBA, Vienna
15 protocols

Kae-Jiun Chang University of California, San Francisco
12 protocols

Reviewer
Manuel D Gahete
  • Post-Doc, IMIBIC - Maimonides Institute for Biomedical Research of Córdoba
Research focus
  • Cancer biology
  • 2 Author merit

Education

PhD, University of Cordoba, 2010

2 Protocols published
Measurement of Free Cytosolic Calcium Concentration ([Ca2+]i) in Single CHO-K1 Cells
Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 10085, Q&A: 0
This is a protocol to analyze the functional response of single CHO-K1 cells to a given treatment in terms of changes in free cytosolic calcium concentration ([Ca2+]i). This is possible by using the Ca2+ indicator dye Fura-2 AM, a polyamino ...
GPCRs Interaction Measurement by Fluorescence Resonance Energy Transfer (FRET)
Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 7330, Q&A: 0
This is a protocol to determine the physical interaction of a G-protein coupled receptor (GPCR) with itself (homodimerization) or with other GPCR (heterodimerazation) using fluorescence resonance energy transfer (FRET). FRET is a distance-dependent ...
7 Protocols reviewed
FRET Reporter Assays for cAMP and Calcium in a 96-well Format Using Genetically Encoded Biosensors Expressed in Living Cells
Authors:  Brandon T. Milliken, Robert P. Doyle, George G. Holz and Oleg G. Chepurny, date: 06/05/2020, view: 701, Q&A: 0
Stimulation of G protein-coupled receptors (GPCR) by hormones and neurotransmitters elicits cellular responses, many of which result from alterations in the concentrations of cytosolic cAMP and Ca2+. Here, we describe a microplate reader ...
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Dual-probe RNA FRET-FISH in Yeast
Authors:  Gable M. Wadsworth, Rasesh Y. Parikh and Harold D. Kim, date: 06/05/2018, view: 4154, Q&A: 0
mRNA Fluorescence In Situ Hybridization (FISH) is a technique commonly used to profile the distribution of transcripts in cells. When combined with the common single molecule technique Fluorescence Resonance Energy Transfer (FRET), FISH can ...
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